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Why there are sticky and blunt end produced in DNA?
sticky ends are more specific and easier to ligate
What is the difference between forceps and blunt probe?
Forceps are instruments that are used to extract the teeth while the blunt probes are used to locate caries.
What can restricted enzymes leave?
Restriction enzymes can leave blunt or sticky ends. This will depend on where they cut the DNA, if they cut at the same point on both strands, they will leave a blunt end, if they cut at different points, they will leave sticky ends. For example: (| represents point of cut) Blunt end ATC|GCTA TAG|CGAT Sticky end A|TCGCTA TAGCGA|T
What are the types of cloning?
In general, sticky end cloning and blunt end cloning
What happens when a restriction enzyme leaves blunt ends?
The restriction enzymes have ability to cut on a specific sequences that are knwon as palandreomic sequences now they cut in two way i.e. blunt and sticky for e.g. if we have a sequece of ATTCGTAAGC it will be cutted in two way as ...................................................TAAGCATTCG 1. ATTCG......... TAAGC i.e., blunt or straight form all ends ....TAAGC .........ATTCG 2. ATT .................CGTAAGC ....TAAGCAT .................TCG i.e., sticky or uneven ends The reason for this phenomena is still not clear or knwon
Which enzymes does not make sticky ends?
Enzymes that do not create sticky ends include blunt-end cutting enzymes such as SmaI and PvuII. These enzymes produce blunt-ended DNA molecules with no overhangs, making them unsuitable for creating cohesive ends for ligation.
In Canada is there a 1965 blunt penny?
Yes. You can tell the difference between a pointed and blunt five because the top of the number is kind of blunt or pointed (if that's not confusing enough :) ). 1965 blunt pennies are worth considerably less than pointed pennies (17 cents in uncirculated condition).
Is there a difference between being frank and being blunt?
One may be frank and honest couched within kindliness and humility. Being blunt is saying something without the regard another's feelings.
How can putting sticky ends onto blunt ended molecules?
Adding sticky ends to blunt-ended molecules involves the use of specific enzymes, such as restriction endonucleases, which create overhanging sequences on DNA fragments. This allows for more efficient ligation during cloning processes, as the complementary sticky ends can base pair with each other, ensuring precise and stable connections between DNA fragments. Sticky ends enhance the specificity and yield of recombinant DNA molecules, making them a preferred choice in genetic engineering and molecular biology applications.
What is the difference between frank and blunt?
Frankness is to reveal oneself from the inner of him/her, without hiding anything. Blunt is a bold statement of agreement or disagreement, without any weightage to the emotions. Frankness involves one person. Bluntness involves two persons. Frankness is friendly to emotions. Blunt does not care emotions
What is the difference in salinity between salt water and fresh water environment?
To be blunt, salt water has a lot more salinity than fresh water.
How can a restriction enzyme leave sticky ends between DNA fragments?
The sticky ends generated by restriction enzymes can easily be joined using an enzyme called ligase. Blunt ends however, cannot be joined so easily. This is why restiction enzymes that create sticky ends are more useful. If blunt ends result, small segments called modifiers are attached to the sticky ends. These modifiers are nucleotide sequences that have sticky ends and attach to the blunt ends, thus making them sticky ends.
