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The restriction enzymes have ability to cut on a specific sequences that are knwon as palandreomic sequences now they cut in two way i.e. blunt and sticky
for e.g. if we have a sequece of ATTCGTAAGC it will be cutted in two way as
...................................................TAAGCATTCG
1. ATTCG......... TAAGC i.e., blunt or straight form all ends
....TAAGC .........ATTCG
2. ATT .................CGTAAGC
....TAAGCAT .................TCG i.e., sticky or uneven ends
The reason for this phenomena is still not clear or knwon
What else can I help you with?
What is the restriction site of the restriction enzyme Hae III?
The restriction site of Hae III is GGCC. It cuts between the G and the C. This produces blunt ends.
What is the restriction site to restriction enzyme Hae III?
Hae III cuts at the site GGCC. It creates blunt ends - meaning a clean cut. This is found between the G and C.
Why do you need to cut the plasmid and cell DNA with the same restriction enzyme?
Cutting both the plasmid and the cell DNA with the same restriction enzyme ensures that they have complementary sticky or blunt ends, allowing for precise ligation. This compatibility is crucial for successful cloning, as it facilitates the insertion of the DNA fragment into the plasmid. If different enzymes are used, the ends would not match, preventing the two DNA molecules from joining effectively. Thus, using the same restriction enzyme increases the efficiency and specificity of the cloning process.
What are steps involved in cohesive and blunt end ligation?
For cohesive end ligation, the steps involve digestion of the vector and insert DNA with compatible restriction enzymes, followed by purification of the digested DNA fragments, mix them together in the presence of ligase enzyme, and transform the ligated DNA into a host organism. For blunt end ligation, the steps are similar with the exception that the DNA fragments are generated by restriction enzymes that produce blunt ends, so there is no need to worry about complementary overhangs.
What is a restiction enzyme?
A restriction enzyme is used to cut DNA (either double or single stranded), cuts can either be "sticky" ( in which cut pieces overlap one another), or "blunt" (in which cut pieces do not overlap). A common rectriction enzyme is EcoR1.
What is the restriction site of the restriction enzyme Hae III?
The restriction site of Hae III is GGCC. It cuts between the G and the C. This produces blunt ends.
What is the restriction site to restriction enzyme Hae III?
Hae III cuts at the site GGCC. It creates blunt ends - meaning a clean cut. This is found between the G and C.
What are steps involved in cohesive and blunt end ligation?
For cohesive end ligation, the steps involve digestion of the vector and insert DNA with compatible restriction enzymes, followed by purification of the digested DNA fragments, mix them together in the presence of ligase enzyme, and transform the ligated DNA into a host organism. For blunt end ligation, the steps are similar with the exception that the DNA fragments are generated by restriction enzymes that produce blunt ends, so there is no need to worry about complementary overhangs.
What is a restiction enzyme?
A restriction enzyme is used to cut DNA (either double or single stranded), cuts can either be "sticky" ( in which cut pieces overlap one another), or "blunt" (in which cut pieces do not overlap). A common rectriction enzyme is EcoR1.
What is Example of restriction enzyme?
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commonly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are:5'-- GAATTC --3'3'-- CTTAAG --5'After this DNA sequence is cut, it might look something like this:5'-- G AATTC --3'3'-- C TTAAG --5'
How can a restriction enzyme leave sticky ends between DNA fragments?
The sticky ends generated by restriction enzymes can easily be joined using an enzyme called ligase. Blunt ends however, cannot be joined so easily. This is why restiction enzymes that create sticky ends are more useful. If blunt ends result, small segments called modifiers are attached to the sticky ends. These modifiers are nucleotide sequences that have sticky ends and attach to the blunt ends, thus making them sticky ends.
How does ligating the plasmid vector and P. putida DNA in the presence of a restriction enzyme increase recombination?
Ligating the plasmid vector and P. putida DNA in the presence of a restriction enzyme increases recombination by generating compatible ends on both the plasmid and the target DNA. The restriction enzyme cuts the DNA at specific sites, producing cohesive (sticky) or blunt ends that can easily anneal. When the plasmid vector and the P. putida DNA are mixed, these complementary ends facilitate the ligation process, allowing for more efficient insertion of the target DNA into the plasmid. This enhances the likelihood of successful recombination events, enabling the creation of recombinant DNA molecules.
What seals the sticky ends of restriction fragments to make recombinant DNA?
These sticky ends, if they two pieces match, they will join together to form a recombinant DNA.
What are three ways that people use restriction enzyme?
Restriction enzymes (REN's) cut or cleve DNA. This cutting process is sequence-specific. Which means, the enzyme does not randomly fragment the DNA (or template strand as it is generally called in many experimental protocols). Instead, the enzyme scans the length of the template until it reaches a specific sequence of 4 - 8 nucleotides. Once this sequence (the restriction site) is reached, the enzyme produces a cut in the template and generates either blunt ends (no overhangs) or sticky ends (having both 3' and 5' overhangs) The primary application of restriction enzymes is is genetic engineering where we want to insert cDNA sequences into plasmids or other vectors.
Does blunt wraps have nicotine?
Yes. Blunt Wraps are accually compressed Tobacco Pulp.
What happens if you eat a blunt?
You can face gastric problems.
What are linkers in genetic engineering?
these are short pieces of double stranded DNA of known nucleotide sequence,its a blunt-ended,but contains a restriction site.
