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What happens when a restriction enzyme is used on DNA?
when restriction enzyme is use on DNA basically it just first losen up the DNA, usally DNA is coiled, and so the restriction enzyme jsut breka the DNA and leave a sticky end, so that it can be put back together, the cell have to be able to do that because in nature, that's the way for cell to stop protein production and the cell still need that gene
How do you draw a plasmid map?
To draw a plasmid map, you first need the plasmid sequence. Then, you can use specialized software like SnapGene or Benchling to input the sequence and generate a visual representation of the plasmid with features like genes, promoters, restriction sites, and other elements. Plasmid maps are typically presented as circular diagrams.
What is one way to determine whether a bacterial culture has received a recombinant plasmid?
The plasmid have a "reporter gene" inside it, generally resistance to specific antibiotic. the plasmid is transformed into bacteria that don't have resistance to that specific antibiotic drug, and than the cultured on a petri-dish that contain the antibiotic drug. Only bacteria that had receive the plasmid will have resistance and grow, all the other will die.
What describes a plasmid?
A piece of DNA transferred by a bacteriumIt is a piece of non-reproductive DNA, typically within a bacterium, that can be transferred to other organisms of the same or different species.
How many copies of one enzyme are in a cell?
Enzymes can have varying concentrations inside a cell. If the need for an enzyme is small then few enzymes will be in the cell however if there a signal to the cell that would cause a drastic need for more enzymes then the production and thus the concentration would increase.
What must researchers know before they begin the process of gentic engineering?
you need to know which restriction enzyme to use. also, who is the doner and the plasmid.
Why must you use an enzyme that will not cut anywhere within the gene that you are inserting into a plasmid?
If you are trying to take a gene from a DNA strand and put insert it into a plasmid, you wouldn't want a restriction enzyme to cut that gene up, or else it would be pretty useless. In other words, you need an enzyme or two that cuts outside that gene so that it can be functional after it's inserted into a plasmid. After your gene of interest is inserted into a plasmid, the plasmid can be put back into a bacterium, then you could genetically engineer plants with it or let the bacterium reproduce and produce many copies of a protein that you had wanted to make in the first place.
What happens when a restriction enzyme is used on DNA?
when restriction enzyme is use on DNA basically it just first losen up the DNA, usally DNA is coiled, and so the restriction enzyme jsut breka the DNA and leave a sticky end, so that it can be put back together, the cell have to be able to do that because in nature, that's the way for cell to stop protein production and the cell still need that gene
How do you draw a plasmid map?
To draw a plasmid map, you first need the plasmid sequence. Then, you can use specialized software like SnapGene or Benchling to input the sequence and generate a visual representation of the plasmid with features like genes, promoters, restriction sites, and other elements. Plasmid maps are typically presented as circular diagrams.
What is one way to determine whether a bacterial culture has received a recombinant plasmid?
The plasmid have a "reporter gene" inside it, generally resistance to specific antibiotic. the plasmid is transformed into bacteria that don't have resistance to that specific antibiotic drug, and than the cultured on a petri-dish that contain the antibiotic drug. Only bacteria that had receive the plasmid will have resistance and grow, all the other will die.
What describes a plasmid?
A piece of DNA transferred by a bacteriumIt is a piece of non-reproductive DNA, typically within a bacterium, that can be transferred to other organisms of the same or different species.
What is the role of BSA in restriction enzyme activity?
BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. This protein does not affect other enzymes that do not need it for stabilization.
How many copies of one enzyme are in a cell?
Enzymes can have varying concentrations inside a cell. If the need for an enzyme is small then few enzymes will be in the cell however if there a signal to the cell that would cause a drastic need for more enzymes then the production and thus the concentration would increase.
What are the common challenges faced when dealing with the restriction mapping problem in genetic analysis?
Common challenges faced when dealing with the restriction mapping problem in genetic analysis include the complexity of the DNA sequence, the presence of repetitive regions, the need for accurate enzyme recognition sites, and the difficulty in resolving closely spaced restriction sites.
Need restriction code for Nokia 6300?
you can get restriction codes for your nokia mobile online sites that provides restriction codes.
What is TOPO cloning vector?
A TOPO cloning vector is a specialized plasmid used in molecular biology for the efficient cloning of DNA fragments. It utilizes a topoisomerase enzyme that facilitates the direct ligation of PCR-amplified DNA fragments into the vector without the need for restriction enzyme digestion or ligation steps. This method allows for rapid and high-efficiency cloning, making it a popular choice for generating recombinant DNA. TOPO cloning is particularly useful for cloning fragments with specific ends, such as those generated by Taq polymerase, which adds a single adenine to the 3' ends of PCR products.
If a cell needed to turn a polysaccharide into monosaccharides it would need to perform what?
It needs to hydrolyze (perform hydrolysis on) the polymer into monomers with an enzyme.
