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when restriction enzyme is use on DNA basically it just first losen up the DNA, usally DNA is coiled, and so the restriction enzyme jsut breka the DNA and leave a sticky end, so that it can be put back together, the cell have to be able to do that because in nature, that's the way for cell to stop protein production and the cell still need that gene

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Which restriction enzyme did you use to cut the DNA?

The restriction enzyme used to cut the DNA was EcoRI.


An enzyme that cuts double-stranded DNA at specific nucleotide sequences?

Such an enzyme is called a restriction endonuclease


Which enzyme opens DNA molecules?

Restriction Enzymes


What is a DNA restriction site?

The restriction site is a sequence of DNA that is recognized by an endonuclease, or a protein that cuts DNA, as a site at which the DNA is to be cut. This cutting happens when restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.


What type of enzyme is used to fragment dna?

Restriction enzymes are used to fragment DNA by cutting it at specific recognition sites. These enzymes are naturally found in bacteria as a defense mechanism against foreign DNA, and are commonly used in molecular biology techniques like restriction enzyme digestion.


Where does the restriction enzyme EcoRI cut in a DNA sequence?

The restriction enzyme EcoRI cuts DNA at a specific sequence of bases, which is GAATTC.


What enzyme cuts and seals DNA?

The enzyme that cuts DNA is called a restriction enzyme, while the enzyme that seals DNA is called DNA ligase. Restriction enzymes cut DNA at specific sequences, creating breaks in the DNA strands, while DNA ligase seals these breaks by catalyzing the formation of phosphodiester bonds between the DNA fragments.


What enzyme is responsible for cutting DNA molecules?

The enzyme responsible for cutting DNA molecules is called a restriction enzyme.


Why restriction enzyme cannot cut its own DNA?

Restriction enzymes are produced by bacteria to help destroy foreign, invading DNA, such as the DNA of bacteriophage (a virus that infects bacterial cells). Every restriction enzyme comes with a methylase enzyme, or more specifically, a DNA methyltransferase. The methylase enzyme methylates (adds a methyl group) to the restriction endonuclease site on the cell's own DNA, which protects the sites from the restriction enzyme so that it does not degrade its own DNA.


Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?

The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.


Hi does any one know if I want to put some restriction enzyme close to cDNA should I put some nucleotide befor this restriction enzyme?

Restriction enzyme cuts DNA strand at specific locations Restriction enzyme cuts DNA strand at specific locations


What does a restriction enzyme do in the process of DNA manipulation?

A restriction enzyme is a protein that cuts DNA at specific sequences, allowing scientists to manipulate and study DNA by cutting it into smaller fragments.