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What else can I help you with?
Explain the role of klenow fragment in recombinant DNA technology?
The Klenow fragment, derived from the DNA polymerase I enzyme, is used in recombinant DNA technology to fill in the single-stranded DNA gaps left in a vector after annealing with a DNA insert. It possesses 5' to 3' polymerase activity and 3' to 5' exonuclease activity, allowing it to extend the DNA strands in a template-directed manner. This helps to create recombinant DNA molecules with high efficiency.
How can a mutation that alters a recognition site be detected by gel electrophoresis?
First, DNA that is mutated and unmutated must be cut with the same restriction enzyme. When these two strains of DNA are run through gel electrophoresis side by side, the mutated DNA will have fewer bands and at least one that does not move as far as the normal DNA. This is because the the restriction enzyme would not cut at the mutated recognition site. The difference in bands in the agarose gel will easily be detected.
What enzyme us used to make radioactively labeled DNA?
DNA polymerase
What enzyme is used to rebuild DNA strands?
My guess is that you're thinking of DNA polymerase.
What is the type of virus that codes backwards from RNA to DNA?
That is called a retrovirus. The enzyme used to code in that direction is called reverse transcriptase.
What kind of molecule is primase?
Primase is an enzyme. It is used to synthesize a short RNA fragment called a primer during DNA replication.
What type of biology do DNA ligase belong to?
DNA ligase belongs to the field of molecular biology, specifically enzyme biology. It plays a crucial role in the process of DNA replication and repair by catalyzing the formation of phosphodiester bonds between DNA strands.
Which restriction enzyme did you use to cut the DNA?
The restriction enzyme used to cut the DNA was EcoRI.
How does double enzyme digestion enhance the efficiency of DNA fragment analysis in molecular biology experiments?
Double enzyme digestion enhances the efficiency of DNA fragment analysis in molecular biology experiments by using two different enzymes to cut the DNA at specific sites, increasing the chances of obtaining the desired fragments. This method allows for more precise and accurate analysis of DNA fragments, leading to better results in experiments.
What does a primer do in PCR and how does it contribute to the amplification of DNA fragments?
A primer in PCR is a short piece of DNA that binds to a specific target sequence on the DNA template. It serves as a starting point for DNA synthesis by the DNA polymerase enzyme. The primer helps the enzyme to accurately copy the target DNA sequence, leading to the amplification of the DNA fragment during PCR.
What is a vector used for in a genetic engineering experiment?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".
Explain the role of klenow fragment in recombinant DNA technology?
The Klenow fragment, derived from the DNA polymerase I enzyme, is used in recombinant DNA technology to fill in the single-stranded DNA gaps left in a vector after annealing with a DNA insert. It possesses 5' to 3' polymerase activity and 3' to 5' exonuclease activity, allowing it to extend the DNA strands in a template-directed manner. This helps to create recombinant DNA molecules with high efficiency.
What enzyme us used to make radioactively labeled DNA?
DNA polymerase
How can a mutation that alters a recognition site be detected by gel electrophoresis?
First, DNA that is mutated and unmutated must be cut with the same restriction enzyme. When these two strains of DNA are run through gel electrophoresis side by side, the mutated DNA will have fewer bands and at least one that does not move as far as the normal DNA. This is because the the restriction enzyme would not cut at the mutated recognition site. The difference in bands in the agarose gel will easily be detected.
What enzyme is used to join DNA nucleotides?
In DNA replication, DNA polymerase III is the enzyme which joins the DNA nucleotides together via phospodiester bonds.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.
What enzyme is used to rebuild DNA strands?
My guess is that you're thinking of DNA polymerase.
59 33 The insertion of a human DNA fragment into a bacterial cell might make it possible for what?
The insertion of a human DNA fragment into a bacterial cell could potentially enable the bacterial cell to produce a human protein or enzyme. This technique is commonly used in biotechnology to produce pharmaceuticals or study gene function.
