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DNA polymerase
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What kind of molecule must be and ldquolabeled and to make radioactively labeled DNA?
To create radioactively labeled DNA, the molecule that must be labeled is a nucleotide. Specifically, one of the nucleotides (adenine, thymine, cytosine, or guanine) can be modified to include a radioactive isotope, such as phosphorus-32 or tritium. When these labeled nucleotides are incorporated into a DNA strand during replication or synthesis, the entire DNA molecule becomes radioactively labeled. This technique is often used in molecular biology for various applications, including tracking DNA synthesis and conducting hybridization experiments.
Can be used to compare the amount of radioactively labeled DNA in two or more samples?
Scintillation counter
Can DNA polymerase be used to determine the general location of radioactively labeled DNA?
Geiger Counter
What reaction is used to radioactively label DNA?
The reaction used to radioactively label DNA is typically performed using a DNA polymerase enzyme along with radioactive nucleotides, such as [α-32P]dNTPs. This allows for the incorporation of the radioactive label into the DNA strand during the polymerase reaction.
What are radioactively tagged bacteriophages to discover that DNA not protein is injected into host cells?
Radioactively tagged bacteriophages are used to confirm that DNA, not protein, is injected into host cells during infection. The radioactively labeled DNA can be detected inside the host cells after infection, providing evidence that DNA is the genetic material transferred by the bacteriophages. This experiment was crucial in establishing DNA as the genetic material in organisms.
What kind of molecule must be and ldquolabeled and to make radioactively labeled DNA?
To create radioactively labeled DNA, the molecule that must be labeled is a nucleotide. Specifically, one of the nucleotides (adenine, thymine, cytosine, or guanine) can be modified to include a radioactive isotope, such as phosphorus-32 or tritium. When these labeled nucleotides are incorporated into a DNA strand during replication or synthesis, the entire DNA molecule becomes radioactively labeled. This technique is often used in molecular biology for various applications, including tracking DNA synthesis and conducting hybridization experiments.
Can be used to compare the amount of radioactively labeled DNA in two or more samples?
Scintillation counter
Can DNA polymerase be used to determine the general location of radioactively labeled DNA?
Geiger Counter
What reaction is used to radioactively label DNA?
The reaction used to radioactively label DNA is typically performed using a DNA polymerase enzyme along with radioactive nucleotides, such as [α-32P]dNTPs. This allows for the incorporation of the radioactive label into the DNA strand during the polymerase reaction.
What are radioactively tagged bacteriophages to discover that DNA not protein is injected into host cells?
Radioactively tagged bacteriophages are used to confirm that DNA, not protein, is injected into host cells during infection. The radioactively labeled DNA can be detected inside the host cells after infection, providing evidence that DNA is the genetic material transferred by the bacteriophages. This experiment was crucial in establishing DNA as the genetic material in organisms.
What of the following reactions is used to radioactively label DNA?
The reaction that is commonly used to radioactively label DNA is the nick translation method, where a DNA molecule is treated with a DNA polymerase, dNTPs (including radioactive ones), and a DNAase to create radioactive labeled DNA fragments.
What can be used to radioactively label DNA?
Synthesis of new DNA
What is laboratory technique in which the body radioactively labeled a substance is mixed with a blood specimen?
The laboratory technique you are referring to is known as radioimmunoassay (RIA). In RIA, a radioactive substance is used to label a specific molecule or antigen, and when this labeled molecule is mixed with a blood specimen containing the corresponding antibody, the level of radioactivity can be used to quantify the amount of antigen present in the blood sample.
What did Hershey and Chase used to radioactively label the T2 phage's proteins?
32P
How is a radioactively labeled probe made?
A radioactively labeled probe is made by attaching a radioactive isotope to a nucleic acid molecule, such as DNA or RNA, which is complementary to the target sequence of interest. This is typically done by incorporating the radioactive nucleotide during the synthesis of the probe or by labeling the probe post-synthesis through various chemical methods. The choice of isotope, such as phosphorus-32 or sulfur-35, depends on the specific application and detection requirements. After labeling, the probe can be used in techniques like hybridization to detect specific nucleic acid sequences in various biological samples.
What molecule is used to make radioactivity labeled DNA?
Radioactive nucleotide
The materials commonly used to shield living beings from potentially harmful radioactively includes?
lead
