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Synthesis of new DNA
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What reaction is used to radioactively label DNA?
The reaction used to radioactively label DNA is typically performed using a DNA polymerase enzyme along with radioactive nucleotides, such as [α-32P]dNTPs. This allows for the incorporation of the radioactive label into the DNA strand during the polymerase reaction.
What of the following reactions is used to radioactively label DNA?
The reaction that is commonly used to radioactively label DNA is the nick translation method, where a DNA molecule is treated with a DNA polymerase, dNTPs (including radioactive ones), and a DNAase to create radioactive labeled DNA fragments.
What is a following reactions that is used to radioactively label DNA?
Synthesis of new DNA.
What enzyme us used to make radioactively labeled DNA?
DNA polymerase
What did Hershey and Chase used to radioactively label the T2 phage's proteins?
32P
What are radioactively tagged bacteriophages to discover that DNA not protein is injected into host cells?
Radioactively tagged bacteriophages are used to confirm that DNA, not protein, is injected into host cells during infection. The radioactively labeled DNA can be detected inside the host cells after infection, providing evidence that DNA is the genetic material transferred by the bacteriophages. This experiment was crucial in establishing DNA as the genetic material in organisms.
Can DNA polymerase be used to determine the general location of radioactively labeled DNA?
Geiger Counter
Can be used to compare the amount of radioactively labeled DNA in two or more samples?
Scintillation counter
What reaction is used to radioactivley label DNA?
The reaction used to radioactively label DNA is the random primer labeling reaction. This involves using short oligonucleotide primers that hybridize randomly to the DNA template, which are then extended using a DNA polymerase in the presence of radioactive nucleotides.
The final step in the hybridization of radioactively DNA and normal DNA is to?
Make a solution with the two types of DNA
How do radioisotopes identify DNA?
Radioisotopes are often used to label DNA molecules for detection purposes. This is achieved by incorporating radioactively labeled nucleotides during DNA replication or using radiolabeled probes that bind specifically to the DNA sequence of interest. By detecting the emitted radiation from the radioisotope, researchers can locate and identify the DNA molecules.
What kind of molecule must be and ldquolabeled and to make radioactively labeled DNA?
To create radioactively labeled DNA, the molecule that must be labeled is a nucleotide. Specifically, one of the nucleotides (adenine, thymine, cytosine, or guanine) can be modified to include a radioactive isotope, such as phosphorus-32 or tritium. When these labeled nucleotides are incorporated into a DNA strand during replication or synthesis, the entire DNA molecule becomes radioactively labeled. This technique is often used in molecular biology for various applications, including tracking DNA synthesis and conducting hybridization experiments.
