Synthesis of new DNA.
The reaction that is commonly used to radioactively label DNA is the nick translation method, where a DNA molecule is treated with a DNA polymerase, dNTPs (including radioactive ones), and a DNAase to create radioactive labeled DNA fragments.
The reaction used to radioactively label DNA is the random primer labeling reaction. This involves using short oligonucleotide primers that hybridize randomly to the DNA template, which are then extended using a DNA polymerase in the presence of radioactive nucleotides.
Radioisotopes are often used to label DNA molecules for detection purposes. This is achieved by incorporating radioactively labeled nucleotides during DNA replication or using radiolabeled probes that bind specifically to the DNA sequence of interest. By detecting the emitted radiation from the radioisotope, researchers can locate and identify the DNA molecules.
The light reactions provide energy carriers for the dark reactions.
Anabolic reactions are chemical processes in the body that build molecules and require energy. Another term used to describe them is biosynthetic reactions.
The reaction that is commonly used to radioactively label DNA is the nick translation method, where a DNA molecule is treated with a DNA polymerase, dNTPs (including radioactive ones), and a DNAase to create radioactive labeled DNA fragments.
Synthesis of new DNA
32P
The reaction used to radioactively label DNA is typically performed using a DNA polymerase enzyme along with radioactive nucleotides, such as [α-32P]dNTPs. This allows for the incorporation of the radioactive label into the DNA strand during the polymerase reaction.
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DNA polymerase
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Scintillation counter
Radioactively tagged bacteriophages are used to confirm that DNA, not protein, is injected into host cells during infection. The radioactively labeled DNA can be detected inside the host cells after infection, providing evidence that DNA is the genetic material transferred by the bacteriophages. This experiment was crucial in establishing DNA as the genetic material in organisms.
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The energy production of the sun & the atomic bombs used in world war ll
The laboratory technique you are referring to is known as radioimmunoassay (RIA). In RIA, a radioactive substance is used to label a specific molecule or antigen, and when this labeled molecule is mixed with a blood specimen containing the corresponding antibody, the level of radioactivity can be used to quantify the amount of antigen present in the blood sample.