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A radioactively labeled probe is made by attaching a radioactive isotope to a nucleic acid molecule, such as DNA or RNA, which is complementary to the target sequence of interest. This is typically done by incorporating the radioactive nucleotide during the synthesis of the probe or by labeling the probe post-synthesis through various chemical methods. The choice of isotope, such as phosphorus-32 or sulfur-35, depends on the specific application and detection requirements. After labeling, the probe can be used in techniques like hybridization to detect specific nucleic acid sequences in various biological samples.
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How is radioactively labeled probe made?
A radioactively labeled probe is created by attaching a radioactive isotope, such as phosphorus-32 or carbon-14, to a nucleic acid molecule (DNA or RNA). This is typically achieved through chemical methods that incorporate the radioactive isotope during the synthesis of the nucleic acid or by enzymatic labeling, where enzymes incorporate labeled nucleotides into the growing nucleic acid strand. The resulting probe can then be used in various applications, such as hybridization assays, to detect specific sequences of nucleic acids. Proper safety protocols must be followed due to the radioactive materials involved.
In an experiment a scientist makes a radioactively labeled probe using yeast DNA she then discovers that the probe hybridizes to a small segment?
In the experiment, the scientist uses a radioactively labeled probe derived from yeast DNA to identify complementary sequences in a sample. The probe hybridizes to a small segment, indicating that this segment contains sequences complementary to the yeast DNA. This hybridization suggests a potential relationship or functional similarity between the yeast DNA and the target segment, which could lead to further investigations into gene function or evolutionary relationships. The results can provide insights into genetic expression or regulatory mechanisms in the organism being studied.
In an experiment a scientist makes a radioactively labeled probe using human DNA. She then discovers that the probe hybridizes?
to complementary sequences in the target DNA, indicating successful binding between the probe and specific regions of the genome. This hybridization confirms the presence of the corresponding sequences in the sample being tested. The labeled probe can be used to visualize or quantify the target DNA, providing insights into gene expression, genetic variations, or the presence of specific mutations in the sample.
What kind of molecule must be labeled to make radioactively labeled DNA?
Radioactive nucleotide
What enzyme us used to make radioactively labeled DNA?
DNA polymerase
What kind of molecule must be and ldquolabeled and to make radioactively labeled DNA?
To create radioactively labeled DNA, the molecule that must be labeled is a nucleotide. Specifically, one of the nucleotides (adenine, thymine, cytosine, or guanine) can be modified to include a radioactive isotope, such as phosphorus-32 or tritium. When these labeled nucleotides are incorporated into a DNA strand during replication or synthesis, the entire DNA molecule becomes radioactively labeled. This technique is often used in molecular biology for various applications, including tracking DNA synthesis and conducting hybridization experiments.
Can be used to compare the amount of radioactively labeled DNA in two or more samples?
Scintillation counter
Can be use to determine the precise amount of radioactively labeled DNA in a chemical solution?
scintillation counter. APEX
Can DNA polymerase be used to determine the general location of radioactively labeled DNA?
Geiger Counter
You briefly expose bacteria undergoing DNA replication to radioactively labeled nucleotides when you centrifuge the DNA isolated from the bacteria the DNA separates into two classes one class of la?
The DNA separated into two classes: labeled DNA and unlabeled DNA. The labeled DNA contains the radioactively labeled nucleotides that were incorporated during DNA replication, while the unlabeled DNA represents the original, non-radioactively labeled DNA from the bacteria. The centrifugation process separated the DNA based on density, with the heavier labeled DNA migrating to a higher position in the centrifuge tube compared to the unlabeled DNA.
Cells of the pancreas will incorporate radioactively labeled amino acids into proteins?
ER golgi vesicles that fuse with plasma membrane
Using radioactively labeled DNA probes in studies of heredity makes it possible for scientist to do what?
Study the inheritance of traits that are not seen as a phenotype
