0
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commonly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are:
5'-- GAATTC --3'
3'-- CTTAAG --5'
After this DNA sequence is cut, it might look something like this:
5'-- G AATTC --3'
3'-- C TTAAG --5'
What else can I help you with?
What is the restriction site of the restriction enzyme TaqI?
TaqI's restriction site is:TCGAAGCT
Why did you cut both segments of DNA with the same restriction enzyme?
You use the same enzyme inn order to get the same restriction and binding sites.
What is the restriction site of the restriction enzyme Hae III?
The restriction site of Hae III is GGCC. It cuts between the G and the C. This produces blunt ends.
What enzyme do scientists use to cut genes out of strands if DNA?
restriction enzymes
Which enzyme opens DNA molecules?
Restriction Enzymes
An enzyme that cuts double-stranded DNA at specific nucleotide sequences?
Such an enzyme is called a restriction endonuclease
Which restriction enzyme did you use to cut the DNA?
The restriction enzyme used to cut the DNA was EcoRI.
Hi does any one know if I want to put some restriction enzyme close to cDNA should I put some nucleotide befor this restriction enzyme?
Restriction enzyme cuts DNA strand at specific locations Restriction enzyme cuts DNA strand at specific locations
Restriction enzyme digestion?
Yes?
Where does the restriction enzyme EcoRI cut in a DNA sequence?
The restriction enzyme EcoRI cuts DNA at a specific sequence of bases, which is GAATTC.
What is the difference between a restriction enzyme and an endonuclease?
A restriction enzyme is a type of endonuclease. Endonucleases are enzymes that cut DNA at specific sequences, while restriction enzymes specifically cut DNA at recognition sites called restriction sites.
Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?
The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.
What enzyme is responsible for cutting DNA molecules?
The enzyme responsible for cutting DNA molecules is called a restriction enzyme.
What is the restriction site of the restriction enzyme TaqI?
TaqI's restriction site is:TCGAAGCT
Why restriction enzyme cannot cut its own DNA?
Restriction enzymes are produced by bacteria to help destroy foreign, invading DNA, such as the DNA of bacteriophage (a virus that infects bacterial cells). Every restriction enzyme comes with a methylase enzyme, or more specifically, a DNA methyltransferase. The methylase enzyme methylates (adds a methyl group) to the restriction endonuclease site on the cell's own DNA, which protects the sites from the restriction enzyme so that it does not degrade its own DNA.
Which enzyme is used to produce RFLPs?
Restriction enzymes.
What tool to use when cutting plasmid?
a Restriction Enzyme
