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Single digest and double digest methods are techniques used in molecular Biology to cut DNA into smaller fragments for analysis.

In single digest, one restriction enzyme is used to cut the DNA at specific recognition sites, resulting in fragments of varying sizes.

In double digest, two different restriction enzymes are used sequentially to cut the DNA at two different recognition sites, resulting in smaller and more precise fragments.

Overall, double digest methods provide more detailed and accurate information about the DNA sequence compared to single digest methods.

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