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The DNA of a human cell can be cut and rearranged by using?
The answer as of Castle Learning was choice 4, Enzymes.
Where are restriction enzymes obtained from?
Restriction enzymes are obtained from many prokaryotes and about 1500 enzymes with known sequence recognition sites have been isolated. Restriction enzyme is a protein that recognize a specific, short nucelotide sequence.
Based on restriction maps of plasmid determine the number of DNA fragments and sizes of the fragments?
Plasmids are circular pieces of DNA, so the number of fragments equals the number of cuts from the restriction enzymes. You can easily see this if you start with one restriction enzyme that cuts the plasmid in only one place. Cutting the circle in one place yields you only one fragment. If the restriction cuts in two places, you end up with two fragments; with three places, three fragments, etc. With linear chromosomes, the situation is different. Cutting a linear chromosome in one place yields two fragments, cutting in two places yields three fragments, etc. So the number of fragments is always one more than the number of cuts. A restriction map of a plasmid will show all of the cuts the restriction enzymes made. Each cut is labeled with the enzyme that made it. One can count the spaces between cuts to determine the number of fragments that are produced. Restriction maps usually (but not always) also show the size of each fragment.
Which enzyme would cut the human DNA shown in Part A on both sides of the vgp gene but not inside the gene?
1. Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene? Answer: BamHI, HaeIII, and HindIII 2. Which enzymes(s) would cut the plasmid without disrupting the function of the amp^R gene? Answer: BamHI, EcoRI, and HaeIII 3. Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid? Answer: BamHI, EcoRI, and HindIII 4. Which one restriction enzyme satisfies all three of the requirements listed above? Answer: BamHI only
What is cDNA-AFLP technique?
Amplified fragment length polymorphism PCR (or AFLP-PCR or just AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990¡¯s by Keygene, AFLP uses restriction enzymes to cut genomic DNA, followed by ligation of complementary double stranded adaptors to the ends of the restriction fragments. A subset of the restriction fragments are then amplified using two primers complementary to the adaptor and restriction site fragments. The fragments are visualized on denaturing polyacrylamide gels either through autoradiography or fluorescence methodologies. AFLP-PCR is a highly sensitive method for detecting polymorphisms in DNA. The technique was originally described by Vos and Zabeau in 1993. The procedure of this technique is divided into three steps: 1. Digestion of total cellular DNA with one or more restriction enzymes and ligation of restriction half-site specific adaptors to all restriction fragments. 2. Selective amplification of some of these fragments with two PCR primers that have corresponding adaptor and restriction site specific sequences. 3. Electrophoretic separation of amplicons on a gel matrix, followed by visualisation of the band pattern. A variation on AFLP is cDNA-AFLP, which is used to quantify differences in gene expression levels. Another variation on AFLP is TE Display, used to detect transposable element mobility.
Can Restriction Enzymes be used on all DNA?
Restriction enzymes cut DNA at sites called restriction sites on the DNA. These restriction sites are specific sequences of 6 - 8 nucleotide bases. Restriction enzymes can be used on all types of DNA. If the DNA is cut by a certain restriction enzyme, then we know that the DNA contained the restriction site. This sort of an experiment is called restriction site analysis
What are all the palindromic numbers?
That's an infinite list.
Why don't bacterial chromosomes get cut out with the restriction enzymes present in the cell?
Bacterial chromosomes are protected from being cut by restriction enzymes because they contain specific DNA sequences called methylated sites that act as recognition markers for the restriction enzymes. These methylated sites prevent the enzymes from cutting the bacterial chromosome by blocking their activity.
Are all palindromic numbers divisible by 11?
No. For instance, 101 is not divisible by 11.
What are the palindromic numbers between 1000 and 5000?
1001,1111,1221,1331,1441,1551,1661,1771,1881,1991,2002,2112,2222,2332,2442,2552,2662,2772,2882,2992,3003,3113,3223,3333,3443,3553,3663,3773,3883,3993,4004,4114,4224,4334,4444,4554,4664,4774,4884,4994: 40 numbers in all.
Is air brake a restriction or endorsement in va?
It is a CDL restriction in all states and territories of the United States.
What is the sum of all 4 digit numbers that are the same when their digits are reversed?
The sum of all palindromic numbers from 1001 to 9999 is 495000.
What three digit number whose digits are all odd and different has a sum of the three digits that is palindromic?
137?
The DNA of a human cell can be cut and rearranged by using?
The answer as of Castle Learning was choice 4, Enzymes.
Which enzyme do scientist use to cut genes out of strands of DNA?
restriction endonuclease
What does a J Restriction mean on a Texas Drivers License?
You must have a driver at least 21 years of age in sight at all times while you're driving.
How do men only dating sites work?
I am not sure there are any men only dating sites. IF you mean gay/lesbian dating sites, all major dating websites have this option for you to choose on them.
