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What is the term that describes the sequence of DNA that a restriction enzyme finds and cuts?
Template Sequence
Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?
The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.
Where is the transcription start site located in the DNA sequence?
The transcription start site is located at the beginning of a gene in the DNA sequence. It is where the process of transcription, which produces RNA from DNA, begins.
What is a DNA restriction site?
The restriction site is a sequence of DNA that is recognized by an endonuclease, or a protein that cuts DNA, as a site at which the DNA is to be cut. This cutting happens when restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.
What determines how DNA will be cut by a restriction enzyme?
Restriction enzymes cut DNA at specific sites called restriction sites. These restriction sites are typically 6 - 8 nucleotides in length and have a defined set of nucleotide bases. For example, the restriction enzyme Eco R1 cuts at the site: AGGTTC. Therefore, if the target DNA contains the above sequence, Eco R1 is able to cut it within the restriction site. Hence, by looking into the target site and which restriction enzymes are being used, on can make an accurate estimate of where the target DNA will be cut
What is the term that describes the sequence of DNA that a restriction enzyme finds and cuts?
Template Sequence
Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?
The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.
What will cut a DNA sequence only if it matches the sequence precisely?
A restriction enzyme will cut a DNA sequence only if it matches the specific recognition sequence of that enzyme. These enzymes are highly specific and will cleave the DNA at a particular site when the target sequence is present in the DNA molecule.
How restriction enzyms are use in DNA recommination research?
Restriction enzymes are used in DNA recombination research to cut DNA at specific recognition sequences. This allows researchers to generate DNA fragments with desired sequences that can be further manipulated or combined with other DNA fragments to create recombinant DNA molecules. By cutting DNA at precise locations, restriction enzymes facilitate the cloning of genes or the construction of genetically modified organisms.
How many lambda DNA fragments does BamHI make?
There are five BamHI cut sites in lambda DNA: Location 5505 22346 27972 34499 41732 So, BamHI will digest lambda DNA into six fragments.
How can bacterium produce restriction enzymes that do not cleave it's DNA?
Restriction enzymes cleave DNA at a particular recognition site -- a particular sequence of nucleotides. You can imagine the following scenarios:1. The bacterial chromosome does not contain the recognition sequence2. The bacterial chromosome contains the recognition sequence, but that particular part of the DNA is either supercoiled to keep the restriction enzyme from finding the sequence, or it's single stranded as when being replicated or transcribed.3. The bacterial chromosome contains the recognition sequence, but that particular part of the DNA is methylated or modified in some other way which prevents the restriction enzyme from attaching.
Where is the transcription start site located in the DNA sequence?
The transcription start site is located at the beginning of a gene in the DNA sequence. It is where the process of transcription, which produces RNA from DNA, begins.
What genetic locus serves as a recognition site for RNA polymerase?
The promoter region, typically located upstream of the coding sequence, serves as the recognition site for RNA polymerase. It contains specific DNA sequences that allow RNA polymerase to bind and initiate transcription.
What are restriction enzymes?
Restriction enzymes (also known as restriction endonucleases) are proteins which cut DNA up at specific sequences in the genome. For example, the commonly used restriction endonuclease EcoRI recognizes every point in DNA with the sequence GAATTC, and cuts at the point between the Guanine and Adenine. Interestingly, the recognition sequences for most restriction endonucleases are genetic palindromes, e.g., the sequence reads exactly the same backwards on the complementary strand. In the case of EcoRI, the two complementary DNA strands for the recognition sequence are: 5'--GAATTC ---3'3'--CTTAAG--5'
Where can images for a DNA sequence be found?
There are three distinct sites on the web were DNA sequence images can be viewed. The first is the U.S. government site Genome. The second is DNA 11, which is a site of personal DNA images. The last site is Universe Review.
What types of DNA sequences do restriction enzymes recognize?
Restriction enzymes recognize specific DNA sequences known as recognition sites, which are typically palindromic and range in length from 4 to 8 base pairs. These enzymes can cleave DNA at these recognition sites, either by cutting between specific bases within the recognition sequence or nearby.
How can a mutation that alters a recognition site be detected by gel electrophoresis?
First, DNA that is mutated and unmutated must be cut with the same restriction enzyme. When these two strains of DNA are run through gel electrophoresis side by side, the mutated DNA will have fewer bands and at least one that does not move as far as the normal DNA. This is because the the restriction enzyme would not cut at the mutated recognition site. The difference in bands in the agarose gel will easily be detected.
