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To prepare bacterial cultures using Luria Bertani broth, follow these steps:

  1. Measure out the appropriate amount of LB broth and pour it into a sterile flask.
  2. Autoclave the LB broth to sterilize it.
  3. Inoculate the sterile LB broth with the bacterial culture.
  4. Incubate the flask at the appropriate temperature for the bacteria being cultured.
  5. Monitor the growth of the bacteria by measuring optical density or performing colony counts.
  6. Use the bacterial culture for experiments or storage as needed.
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5mo ago

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