you could dry out the cell under the light, which will make them no longer viable. So if you want to study living cells, wet mounts are necessary.
In wet mount preparations living activities inside cell can be observed under magnifications.
it looks beter,
and it doesent freeze.
the major advantage of using a living cell preparation rather than a stained one is that it allows you to observe motility in the bacterium.
well because its better
You can view their motility.
jj
There are many disadvantages to stain and not staining cells before viewing them. One disadvantage of gram staining is that the cells cannot be alive while being stained, so viewing live stained cells is impossible.
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
Chromatin coils and condenses to form deeply-staining bodies in prophase. This is the first stage of mitosis, the process in which cells divide to create new cells.
Most cells contain structures that are transparent and without color, which makes visualization difficult, even with the aid of a microscope. Cell staining is used to gain more information about cells and tissues, and allows for the structures within cells to be seen in great detail
If your talking about Acid Fast staining (aka Ziehl-Neelsen staining), after the addition of the primary stain (carbol fuchsin) heat is applied in order to facilitate proper staining. Bacteria such as Mycobacterium contain large amounts of lipid substances within their cell wall called mycolic acids. These acids resist staining by ordinary methods such as gram staining (where heat is not applied after primary staining). On application of heat, the stain carbol fuschin penetrates the cell wall and stains the cells pink. Following the secondary staining (methylene blue) the acid fast positive cells appear pink while others are stained blue. Endospore staining is yet another staining technique where heat is applied after primary staining (malachite green). In this case the spores are impermeable to stains, hence heating favours proper permeation of stain. Endospores appear green while vegetative cells appear red (secondary stain saffranine). Not all staining procedures involve applying heat after primary staining. However, heat is applied before even beginning the staining procedure. This is called heat fixing, where the cells are fixed so that they are not washed away during the subsequent washing steps.
Advantages: easy to carry out, natural shape and side Disadvatages: heat fixing can distorte cells, more attention needed
There are many disadvantages to stain and not staining cells before viewing them. One disadvantage of gram staining is that the cells cannot be alive while being stained, so viewing live stained cells is impossible.
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
Tattoo artist. (BTP)
they are easier to see when you use a microscope
to kill cells
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
what are the advantages and disadvantages primary cells?
There are several uses for a staining jar. In microscopy, it is used for staining tissues and cells for slides. After being stained with dyes or stains, the specimens can also be placed in the jar to look for certain aspects.
Chromatin coils and condenses to form deeply-staining bodies in prophase. This is the first stage of mitosis, the process in which cells divide to create new cells.
The combination of Congo red cream and distilled water is used for staining cells for viewing under the microscope. Staining make the individual cells much easier to see.
It will make them stand out more under a microscope