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What Name three basic stains?

Updated: 8/10/2023
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13y ago

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Bacterial staining: These are the methods for staining various parts of bacterial cell.

1. Gram staining: Used for primary identification of two major groups of bacteria - Gram Positive and Gram Negative.

2. Cell wall staining: shows cell wall of bacteria.

3. Nuclear staining: Though bacteria do not possess true Nucleus, an area high concentration of Nucleic acids can be stained.

4. Lipid stain

5. Spore stain

6. Flagella

7. Capsule

8.Meta chrimatic granules.

9.. Spirocheate staining: this method is used to show presence of spirocheates in a smear.

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16y ago
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14y ago

stains are salt of a +ve and -ve ion one of which is coloured and is known as chromophore. stains are frequently used in Biology and medicine to highlight structures in biological tissues for viewing often with the aid of microscope. Stains may be used to define and exammine bulk tissues, cells populations or organelles within individual cells. stains are generally classified on the basis of ion to which they intract as acidic, basic and neutaral. e.g. methylene blue, eosin, basic fuschin, malachite green etc.

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14y ago

Stains are salt composed of a (+)ve and a (-)ve ion one of which is coloured and is known as chromophore.

In which dye colour is in the (+)ve ion is called Basic dye & in Acidic dye colour is in the (-)ve ion.

Basic dyes:

Methyleneblue, crystal violet, Safranin, Malachitegree etc. are the basic dye which bind to negatively charged organ such as nucleic acid &protein .

Acidic dyes:

Eosin , acid fuschinetc. etc.are the acidicdye which bind to

Positively charged cell structures.


Common biological stains

• Crystal violet:

when combined with a suitable mordant, stains cell walls purple. Crystal violet is an important component in Gram staining

• Eosin

used as a counterstain. imparting a pink or red colour to cytoplasmic material, cell membranes, and some extracellular structures. It also imparts a strong red colour to red blood cells.

• Ethidium bromide:

intercalates and stains DNA, providing a fluorescent red-orange stain

• Fuchsin

used to stain collagen, smooth muscle, or mitochondria Iodine

used as an indicator for starch. When starch is mixed with iodine in solution, an intensely dark blue color develops, representing a starch/iodine complex.

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11y ago

The three basic stains are:

1. crystal violet

2. carbolfuchsin

3. methylene blue

These are basic stains because they have the ability to bind with the bacteria using ionic interactions. This means that the stains have postively charged groups that bind with the bacteria's negatively charged groups.

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16y ago

Stains are dirty marks that are not easily removed.

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13y ago

crystal violet, methylene blue, and safranin

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12y ago

Crystal violet

Methylene blue

Safranin

We just did this today in honors bio 2 hope it helps.

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12y ago

Methylene Blue, Crystal Violet, and carbon fuhsion

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