The Advantages
A high-power objective lens is that it provides the most magnification possible with most standard, monocular (single) eyepiece microscopes. It is what's required to provide the most magnification -- that is, to get as close as possible to the specimen being examined.
The Disadvantages
The field of view decreases which means you see less of what you're observing. Also, what you're observing can turn out unfocused and sort of blurry.
Advantages: High resolution for imaging at the nanoscale, ability to visualize subcellular structures in detail, and can be used to study materials at atomic level. Disadvantages: Expensive equipment and maintenance costs, samples may require specialized preparation techniques, and can be time-consuming to acquire and interpret data.
The fine adjustment knob is used with the power objective on the microscope. Since the objective is already at the highest objective possible for that microscope, using the coarse adjustment knob would focus to much on the object making the details harder to read. Using the fine adjustment knob, you are able to see the fine details within the object or organism you are studying. The higher the power, the finer the knob and the lower the objective, the coarser the knob and objective.
The coarse focus knob should be used with the low power objective but not with the high power objective. The point of using the coarse focus knob is to bring the specimen into general focus before fine-tuning with the high power objective, which requires more precise adjustments.
Reducing the light when using a 10x objective lens can help prevent overexposure of the sample, which can lead to image distortion and loss of detail. It can also improve contrast and image quality by ensuring that the light intensity is appropriately balanced for the higher magnification provided by the 10x lens.
Advantages - 1) there are 2 eyepieces which means you don't have to strain your eyes so much and makes it easier to focus on the object you are looking at. 2) objects can be looked at in 3D which makes it easier and more interesting to assess the object. 3) it is able to magnify so closely that you can see objects that cannot be seen using other microscopes. Disadvantages compared to current miscroscopes: None.
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disadvantages *not to scale *there are limitations
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