The fine adjustment knob is used with the power objective on the microscope. Since the objective is already at the highest objective possible for that microscope, using the coarse adjustment knob would focus to much on the object making the details harder to read. Using the fine adjustment knob, you are able to see the fine details within the object or organism you are studying. The higher the power, the finer the knob and the lower the objective, the coarser the knob and objective.
Fine adjustment knob because otherwise you risk cracking your slide because your lens is very very close to specimen.
Fine focus knob.
Using high power objective lense
The high power objective in a microscope is the largest lens in the microscope.
Using high power objective lense
what magnification is ontained with the high power objective
Might add extra, but i really hope this helps! scanning objective - for locating the specimen on the slide (= low power objective) high power objective - magnifies the specimen to provide a detailed image coarse adjustment - used to focus the image when using low power diaphragm - used to adjust the amount of light passing through the specimen revolving nosepiece - holds the three objective lenses - it can be rotated to change the objective in use arm - holds the stage and the lens system - can be used to tilt the microscope (but not advised) substaGe lamp - provides a uniform illumination (more reliable than daylight !!)
All the threads can be in focus again using the high-power objective if a certain thread is focused using the control knob to make adjustments. The knob allows for very small changes to be made which will create a precise focus.
THE disadvantage of using high power objective is to
First focus the microscope on the lowest objective using first the coarse djustment knob and then the fine adjustment knob. Once you have it in focus, move on and do the same for the next highest objective. Continue this process until you reach the highest objective and focus it in the same fashion.
Because you are at the highest magnification, only turn the fine adjustment knob when you are using the high-powered objective lens, and not the coarse adjustment. Otherwise you risk getting your sample out of focus, and you will probably only be able to refocusby starting with the lowest power objective lens.
using the course adjustment to focus the specimen under high power
Using high power objective lense
Using high power objective lense
The high-power objective magnifies the image 4x.
The high power objective in a microscope is the largest lens in the microscope.
Using high power objective lense
The primary rule to apply when using the high power objective is to always stay focused. By doing this, a person or team will be able to reach their goal much easier.
On a typical light microscope, the low power objective lens will be shorter than the high power objective lens.