Safranin is a biological stain which generally colors the cell nuclei red. In analytical chemistry, it is also used as redox indicator.
you can omit the last step - the safranin counterstain
the counter stain is safranin 0.5%
Safranin
safranin
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
safranin is a biological stain used in histology n cytology
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
you can omit the last step - the safranin counterstain
the counter stain is safranin 0.5%
Safranin
Most likely nothing. The gram stain will only stain the peptidoglycan cell wall of bacteria. Most viruses have a protein coat called a capsid. The capsid will not take up either the crystal violet or the safranin of a Gram stain.
We used safranin on how to essilly see the specimen.
We used safranin on how to essilly see the specimen.
safranin
Sure, any basic stain can be used for simple, direct staining.
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
If you are talking about a Gram Stain, then red. E. coli is Gram negative which means that Safranin will stain it red during a gram stain.