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For the capsule stain Congo red or a Nigrosin solution can be used. Next, Maneval's stain is used.
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∙ 15y ago
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∙ 11y agoCopper Sulfate
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How is the capsule stain used in clinical microbiology?
Demonstrating the presence of a capsule is a means of diagnosis and determining the organisms virulence.
What is the appearance of capsule after being stain with capsule stain procedure?
capsule does not gets stained it appears to be colourless when stained using manewals staining procedure
What is the other name for Negative staining?
Capsule Stain
A stain that does not penetrate cell use to color background?
capsule
Does a micrococcus luteus is positive for capsule stain?
yes as it have capsule it give positive test for capsule staining when stain with malachite green The problem with the above answer is that a capsule stain is NOT done with malachite green.
How is the capsule stain used in clinical microbiology?
Demonstrating the presence of a capsule is a means of diagnosis and determining the organisms virulence.
What is the appearance of capsule after being stain with capsule stain procedure?
capsule does not gets stained it appears to be colourless when stained using manewals staining procedure
What is the purpose of emulsifying the bacteria in the capsule stain?
to stain the outside and the inside of the cell.. so the capsule appears transparent
What is the function of copper sulfate in the capsule stain?
The copper sulfate is used as a decolorizing agent rather than water. The copper sulfate washes the purple primary stain out of the capsular material without removing the stain bound to the cell wall. At the same time, the decolorized capsule absorbs the copper sulfate, and the capsule will now appear white in contrast to the deep purple of the cell.
Explain the function of Copper Sulfate in the spore staining procedure?
From what i read in my book: Because the capsule is nonionic, unlike the bacterial cell, the primary stain adheres to the capsule without binding to it. Since the capsule is water- soulube, copper sulfate, rather than water, is used to wash the purple primary stain out of the capsular material without removing the stain that is bound to the cell wall.
What is the slime layer that surround gram negative bacteria and keeps them from taking up purple stain?
capsule
What is the other name for Negative staining?
Capsule Stain
A stain that does not penetrate cell use to color background?
capsule
What materials do you need for a time capsule in ancient Egypt?
Capsule, shovel, dirt, and hole to put it in
What materials are stain and wrinkle resistant?
Silk is stain and wrinkle resistant.
Does a micrococcus luteus is positive for capsule stain?
yes as it have capsule it give positive test for capsule staining when stain with malachite green The problem with the above answer is that a capsule stain is NOT done with malachite green.
What is the chemical composition of bacterial capsule?
It usually consists of polysaccharides,[1] but can be composed of other materials (e.g., polypeptide in B. anthracis). Because most capsules are water soluble[citation needed], they are difficult to stain using standard stains because most stains do not adhere to the capsule. For examination under the microscope, the bacteria and their background are stained darker than the capsule, which doesn't stain. When viewed, bacterial cells as well as the surface they are on, are stained dark, while the capsule remains pale or colorless and appears as a ring around the cell.
