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During DNA replication, the lagging strand is replicated ~1000 (E. coli) base pairs at a time, forming numerous "Okazaki fragments".

Okazaki fragments form because polymerase is only able to replicate DNA in one direction, but DNA is double stranded, with the strands running anti parallel (in opposite directions). The polymerase waits for a region of DNA to be unwound, and while the leading strand is replicated continuously, on the lagging strand the polymerase waits until a region of single stranded DNA is produced before replicating it. This discontinous replication forms the Okazaki fragments, which can then be joined together by ligase (although a different polymerase enzyme, pol I in E. coli, is needed as well to replace the RNA primers with DNA).

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Q: What are the short segments produced in the formation of the 3'-5' strand of DNA called?
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What enzyme used to join the DNA strand that is replicated in small segments called?

DNA polymerase is what I think you are referring to. It will join free nucleotides into a strand based off of a model template.


What fragments of DNA produced from the lagging strand that must be joined called?

Okazaki fragments


What are the small segments synthesized in the lagging strand of DNA?

Okazaki fragments


What are coding segments?

"Coding segments" is the term given to genes, segments of the DNA strand that code for a protein. Much of an organism's genome is non-coding segments, portions that do not have a role in protein synthesis.


Short segments of newly synthesized DNA are joined into a continuous strand by?

ligase


Where does ssb protein bind on lagging strand or on leading strand?

ssb protein bind to the lagging strand as leading strand is invovled in dna replication and lagging strand is invovled in okazaki fragment formation


What strand of mRNA would be produced from the strand of DNA shown below apex.com?

GCT AT


How are transcription and replication differ?

When RNA is produced from DNA strand it is called transcription and when another DNA molecule comes out from the previous one it is called replication


What strand of DNA would be would be produced from the template strand of DNA shown?

Ttg ga


Why is the leading strand considered continuous?

The leading strand is the correct orientation, so it can be replicated continuosly - meaning the DNA Polymerase can continue to add new nucleotides without stopping. New DNA strands can only be created in a 5' to 3' direction. This is different to the lagging strand - which must be looped and copied in small, non-continuos segments. These segments are known as Okazaki fragments.


At each replication fork one new strand of DNA is made of many small pieces. what is the strand of DNA called?

When the two parent strands of DNA are separated to begin replication, one strand is oriented in the 5' to 3' direction while the other strand is oriented in the 3' to 5' direction. DNA replication, however, is inflexible: the enzyme that carries out the replication, DNA polymerase, only functions in the 5' to 3' direction. This characteristic of DNA polymerase means that the daughter strands synthesize through different methods, one adding nucleotides one by one in the direction of the replication fork, the other able to add nucleotides only in chunks. The first strand, which replicates nucleotides one by one is called the leading strand; the other strand, which replicates in chunks, is called the lagging strand. The lagging strand replicates in small segments, called Okazaki fragments. These fragments are stretches of 100 to 200 nucleotides in humans (1000 to 2000 in bacteria).


Name of the DNA strand which is copied to make mRNA?

This is typically called the template DNA, which is the anti-sense strand of DNA. The strand that is not transcribed is called the sense strand.