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What fragments of DNA produced from the lagging strand that must be joined called?
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∙ 11y ago
Okazaki fragments
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∙ 11y ago
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What is lagging strand in replication of DNA?
The two strands of DNA in animal cells are arranged backwards to each other - the start of one is paired with the ending of the other. However, the enzyme that replicates DNA (DNA polymerase) can only work from start to finish. On one strand, DNA polymerase can work front to back in a continuous chain - the strand that allows this is called the leading strand because it "leads" in completion status. On the other strand, the DNA polymerase has to work backwards in pieces and then put the pieces back together into a single chain - the strand that causes this is called the lagging strand because it "lags behind" the other in completion status.
What are the short segments produced in the formation of the 3'-5' strand of DNA called?
During DNA replication, the lagging strand is replicated ~1000 (E. coli) base pairs at a time, forming numerous "Okazaki fragments".Okazaki fragments form because polymerase is only able to replicate DNA in one direction, but DNA is double stranded, with the strands running anti parallel (in opposite directions). The polymerase waits for a region of DNA to be unwound, and while the leading strand is replicated continuously, on the lagging strand the polymerase waits until a region of single stranded DNA is produced before replicating it. This discontinous replication forms the Okazaki fragments, which can then be joined together by ligase (although a different polymerase enzyme, pol I in E. coli, is needed as well to replace the RNA primers with DNA).
Which of the following statements best explains why the leading and lagging strands are synthesized using two different mechanisms?
The leading strand is created continuously, but the lagging strand is created as small fragments, known as Okazaki fragments. These fragments are later joined together to form one complete strand.
What is a okazaki fragment?
Okazaki fragments are only used on the Lagging strand (the one going on 5' to 3' direction) never on the leading one. In fact all that the leading strand uses is the helicase to unwind DNA and DNA polimerase III to form the complementary strand allways in 3' to 5' (the leading strand) direction. The big problem of the laggind strand and the reason that Okazaki fragments exist and all other complementary DNAs (polimerace I, ligase, SSB, primace) is that it runs from 3' to 5'. Now what Okazaki fragments are, is temporary pieces of complementary DNA (iniciated by a primer)that are not joined together, but that later on before it goes back to the helix form will be joined togather by DNA ligase. Here I attache a link to a flash video that will make u understand better! http://www.mcb.harvard.edu/Losick/images/TromboneFINALd.swf
Describe the significance of Okazaki fragments?
Okazaki fragments are created during DNA replication because DNA Polymerase can only add nucleotides in a 5' to 3' direction. This means that one strand (the leading strand) can be continuously created, but the other strand (the lagging strand) runs in the opposite direction. This means that loops must be created and shorter parts of DNA replicated one at a time. This creates fragments on the lagging strand. The RNA primers on this strand are later replaced with DNA by DNA Polymerase I, and joined together with DNA ligase.
What stand of DNA replicated in okasaki fragments?
The lagging strand of DNA contains okazaki fragments, which are later joined by DNA ligase
At each replication fork one new strand of DNA is made of many small pieces what is the strand of DNA?
The lagging stand~Brainly
What is lagging strand in replication of DNA?
The two strands of DNA in animal cells are arranged backwards to each other - the start of one is paired with the ending of the other. However, the enzyme that replicates DNA (DNA polymerase) can only work from start to finish. On one strand, DNA polymerase can work front to back in a continuous chain - the strand that allows this is called the leading strand because it "leads" in completion status. On the other strand, the DNA polymerase has to work backwards in pieces and then put the pieces back together into a single chain - the strand that causes this is called the lagging strand because it "lags behind" the other in completion status.
What are the short segments produced in the formation of the 3'-5' strand of DNA called?
During DNA replication, the lagging strand is replicated ~1000 (E. coli) base pairs at a time, forming numerous "Okazaki fragments".Okazaki fragments form because polymerase is only able to replicate DNA in one direction, but DNA is double stranded, with the strands running anti parallel (in opposite directions). The polymerase waits for a region of DNA to be unwound, and while the leading strand is replicated continuously, on the lagging strand the polymerase waits until a region of single stranded DNA is produced before replicating it. This discontinous replication forms the Okazaki fragments, which can then be joined together by ligase (although a different polymerase enzyme, pol I in E. coli, is needed as well to replace the RNA primers with DNA).
Which of the following statements best explains why the leading and lagging strands are synthesized using two different mechanisms?
The leading strand is created continuously, but the lagging strand is created as small fragments, known as Okazaki fragments. These fragments are later joined together to form one complete strand.
Can DNA fragments from human and bacteria be joined?
Yes, DNA SEGMENTS from humans and bacteria are joined by Enzymes
What is a okazaki fragment?
Okazaki fragments are only used on the Lagging strand (the one going on 5' to 3' direction) never on the leading one. In fact all that the leading strand uses is the helicase to unwind DNA and DNA polimerase III to form the complementary strand allways in 3' to 5' (the leading strand) direction. The big problem of the laggind strand and the reason that Okazaki fragments exist and all other complementary DNAs (polimerace I, ligase, SSB, primace) is that it runs from 3' to 5'. Now what Okazaki fragments are, is temporary pieces of complementary DNA (iniciated by a primer)that are not joined together, but that later on before it goes back to the helix form will be joined togather by DNA ligase. Here I attache a link to a flash video that will make u understand better! http://www.mcb.harvard.edu/Losick/images/TromboneFINALd.swf
The primary function of DNA ligase is to?
The primary function of DNA ligase is to reassemble DNA fragments produced by the use of restriction enzymes. The pieces are joined using a process called ligation, which uses the enzyme, DNA ligase which acts as a molecular 'stapler'. The fragments are joined as follows: - sticky-ended DNA fragments are created from two DNA sources using the same restriction enzyme. - The DNA from the two sources is mixed and complementary ends allowed to form complementary base pairs. This process forming loose and temporary join by hydrogen bonding is called annealing. - DNA ligase is then added to form a permanent link.
Does Fragments of DNA from two different organisms have been joined to form a genome?
yes
Describe the significance of Okazaki fragments?
Okazaki fragments are created during DNA replication because DNA Polymerase can only add nucleotides in a 5' to 3' direction. This means that one strand (the leading strand) can be continuously created, but the other strand (the lagging strand) runs in the opposite direction. This means that loops must be created and shorter parts of DNA replicated one at a time. This creates fragments on the lagging strand. The RNA primers on this strand are later replaced with DNA by DNA Polymerase I, and joined together with DNA ligase.
What type of chemical reactions do ligasis catalyze?
Assuming you mean "ligases," a ligase is an enzyme that catalyzes the formation of a chemical bond between two smaller molecules to form a larger one. The most common example is DNA ligase, which joins DNA strands together. This occurs on the lagging strand of DNA synthesis where synthesis occurs in short Okazaki fragments. These fragments are joined together by ligase, which acts to "seal the nicks" forming one continuous strand of DNA.
Okazaki fragments are joined together by?
During DNA replication Okazaki fragments are joined together by DNA polymerase. Remember that Okazaki fragments start with an RNA primer so RNAse H is need to remove the primer follwed by DNA plymerase to add nucleotides and finally DNA ligase to seal the single strand nick.
