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What aspect of microbial properties can be observed via stab inoculation technique?
Wiki User
∙ 14y ago
The motility of a microorganism can be observed. Motile organisms, using the flagellum, will move away from the stab line, hence will appear to have "diffused" into the medium. Non-motile organisms will remain in the stab line.
Wiki User
∙ 14y ago
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What is properties of image observed under dissecting microscope?
properties of the image under dissecting microscope
If sim medium was used for motility determination for Proteus vulgaris what noticeable change to the medium will be observed?
It will turn black in the inoculation stab as well as throughout the medium because P. vulgaris is a flagellated and motile organism.
Whats the difference between inoculation and contamination?
Five I's 1. Inoculation: The sample is placed into a container of sterile medium that provides microbes with the appropriate nutrients to sustain growth. 2. Incubation: An incubator can be used to adjust the proper growth conditions of a sample. 3. Isolation: The end result of inoculation and incubation is isolation of the microbe. 4. Inspection: The cultures are observed for obvious growth characteristics that could be useful in analyzing the specimen contents. 5. Identification: Determine the type of microbe, usually to the level of species.
What are the differences between streak plate technique and pour plate technique?
Streak Plate:Pure colonies of bacterial or other microorganisms can be obtained on petri dishes by streak plate. The microbial mixture is transfered to the edge of an agar plate with an inoculating loop or swab and then streaked out over the surface in one of several patterns. After the first sector is streaked in dish, the inoculating loop is sterlized and an inoculum for the second sector is obtained from the first sector. The same is done for third and fourth sector. Thus this is a dilution process. Eventually, very few cells will be on inoculating loop, a single cells will drop from it as it is rubbed along the agar surface. These develop into seprate colonies.Pour Plate:Extensively used with procaryotes and fungi, a pour plate also can yield isolated colonies. The original sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating. Then small volumes of several diluted samples are mixed with liquid agar that has been cooled to about 45oC and the mixture are poured immediately into sterile culture dishes. Most bacteria and fungi are not killed by a brief exposure to the warm agar. After the agar has hardened each cell is fixed in place and forms an individual colony.
Measuring bacterial growth?
Bacterial growth can be measured by different ways: The most obvious way to determine microbial numbers is through direct counting. Using counting chamber like PETROFF-HAUSSER counting chamber and HEMOCYTOMETER ( it is also used for eukaryotic cells). The recent counting technique is flow cytometer, in this bacterial suspension is forced through the a small hole or orifice in the Coulter counter chamber and an electric field is applied through the hole and electrodes are placed on both sides of the orifice every time the microbial cell pass will cut the potential and electrical resistance will be noted, which give the number of cells. Onether technique is the membrane filter technique in which the sample is first filtered through a black polycarbonate membrane filter. Then bacteria are stained with a flouroscent dye such as acridine orange, and observed microscopically. The stained cells are easily observed against the black background of the membrane filter and can be counted when viewed with an epifluorescense microscope. Bacterial growth can also be measured by colony counting method.
Properties can be observed without chemically changing matter?
physical properties can be observed without chemically changing matter
Name 5 physical properties of matter that can be observed?
There are many different physical properties of matter that can be observed. Consistency, color, and texture are just 3 properties.
What condition can chemical properties be observed?
Chemical Properties can be observed only when substances in a sample of matter are changing into different substances.
Can the chemical properties of a substance be observed without changing the substance?
physical properties
What Properties Can Be Observed Without Changing A Substance's Chemical Compositionion.?
All physical properties.
What chemical properties that can be observed?
every thing around you.
What conditions may change observed properties?
enthalpy
What is properties of image observed under dissecting microscope?
properties of the image under dissecting microscope
How does properties can be observed only when the substances in a sample of matter are changing into different substances?
Chemical properties
What do physical properties means?
Physical properties of matter can be observed and tested. They include properties such as color, length, volume, odor, and density.
Why do we study physical properties first?
Physical properties can be observed or measured without changing the composition of matter.
Are properties that can be observed or measured without changing a substance into a new substance?
Yes, these are thy physical properties of substances.
