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stain
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Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?
The counter stain used in the Gram stain procedure is typically safranin or basic fuchsin, which stains Gram-negative bacteria pink or red. In the acid-fast stain procedure, the counter stain used is typically methylene blue or brilliant green, which stains non-acid-fast bacteria blue or green, allowing acid-fast bacteria to retain the primary stain color (carbolfuchsin).
What prevents the cell from appearing green in the finished endospore stain?
The endospore stain uses malachite green, but this dye is rinsed off the cell during the staining procedure. The endospore itself retains the green color due to its resistance to decolorization, making it appear green against a contrasting counterstain like safranin.
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
What is the Dorner endospore stain?
The Dorner endospore stain is a technique that involves using malachite green and safranin dyes to identify endospores in bacterial cells. Endospores are a dormant form of certain bacteria that are resistant to harsh conditions. The stain helps visualize endospores as green structures against a pink background.
Do plants absorb or reflect green light?
YES. Plants absorb AND reflect green light. Leaves are green due to a compound called chlorophyll. The light absorbed by chlorophyll is used to power photosynthesis, the conversion of light energy into chemical energy. On a relative basis, chlorophyll absorbs more blue and red light, compared to green light; and therefore reflects less red and blue light. As a result, there is more green light than red or blue light reflected, so the chlorophyll containing part of the plant appears green. It is commonly thought that chlorophyll does not absorb green light, but that is a fallacy. A dark green leaf can absorb 90% of the green light impinging on it. In contrast, 95% of the red and blue light may be absorbed.
Can you stain green treated wood?
Yes, you can stain green treated wood, but it is recommended to wait for the wood to dry completely before applying the stain.
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Bacterial endospores stain green in the Schaeffer-Fulton spore stain due to the malachite green dye used. This technique is commonly used to visualize endospores in bacteria.
Can you stain green pressure treated wood?
Yes, you can stain green pressure treated wood, but it is recommended to wait for the wood to dry completely before applying the stain. The green color of the wood is due to the treatment process and will fade over time, allowing the stain to penetrate and adhere better.
If applying a green stain over a red stain what colour would I get?
You would get the color grey
What are the two techniques used to visualize endospores?
The crystal violet method and the Schaeffer-Fulton method
A white baseball jersey turned green when washed with socks How do you get it white again without bleaching the green jersey number?
you get the stain out by antieoxden it will take the stain out
What do you get when you mix green and white colors?
You get light green.
How does the malachite green stain enter an endospore?
It is heated.
Are nigrosin and malachite green a functional analogous pair of stain?
No
What dye is used to penetrate and stain an endospore?
Malachite green
What are the properties that differentiate the spore from the vegetative cell?
In the spore stain malachite green is used as a primary stain. This is driven into the cell by heat because of the impermeability of the spore. The stain is allowed to sit for 30 mins to make sure it gets in to the endospores.The stain is then washed and counterstained with safranin red. The endospores retain the green colour from malachite green and of course appear green under the microscope. Whereas the vegetative cells will appear red.
Why is the green light green?
Because a green light has a light bulb behind green plastic.
