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Methylene blue reduction is the method used to measure the bacterial density in milk. The test is evaluated in term of the amount of time required from introduction of the blue until the milk turns white.

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Q: What food can methylene blue be used in to detect bacteria quickly?
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How would the pH of the stain methylene blue affect the staining of bacteria?

the ph of the stain on the bacteria caused by methylene blue would not affect it a lot since all methylene blue is supposed to do is make it visible on the microscope for e.g.


Why methylene blue inhibits gram positive bacteria?

because it is acidic?


Can Methylene blue used to stain bacteria?

Yes because Methylene Blue is a symple stain which allows the staining of Cocci. The only thing that is done with the stain is to show the morphology of the bacteria, so one could tell the shape, size, and, arrangement.


What are the EMB test results for Staphylococcus epidermidis?

The Eosine Methylene Blue test is selective for only gram-negative bacteria. It is possible for gram-positive bacteria to grow, but it would barely grow. Staphylococcus are gram-positive spherical bacteria. So it would not grow on the Eosine methylene Blue Agar.


Why do gram positive bacteria retain the primary stain methylene blue while gram negative bacteria are decolorized by rinsing with ethanol?

capsule


What is the behavior of methylene blue towards the animal charcoal?

i think the methylene blue will be make aqua blue because the charcoal will penerate the color of methylene blue,,,there are absorption process,,,in the charoal between the methylene blue.... (kharlz)


Will staph grow on EMB agar?

No, it will not. Methylene blue agar is used to identify gram negative bacteria, staphylococcus is gram positive.


Why is methylene blue used as an indicator of cell respiration?

because methylene blue turns colourless when it is reduced by hydrogen. during respiration hydrogen is produced and instead of reducing NAD, it reduces methylene blue and turns methylene blue colourless. if methylene blue goes from blue to colourless then this shows that the cell is respiring as it is producing a suffiecient amount of hydrogen to decolourise methylene blue


Which of the availble stain enhance your observation the most methylene blue or iodide?

Methylene Blue


Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?

# The pH will determine if the bacteria will have a particular charge. If the chromophore is a positive ion like the methylene blue in the equation shown in the reading, the stain is considered a basic stain; if it is a negative ion, it is an acidic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the negative charges of the bacterial cell.


What color is simple staining on a bacillus and coccus using methylene blue stain?

Methylene blue stains everything blue.


How does methylene blue affect the paramecium?

The methylene blue will make the paramecium very horny and secrete sperm.