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4000 - 6000 x g for a given period of time (depending on density, volume, etc.) is sufficient.
I'm not sure of the maximum rcf, but I've read of some people using 8000 x g. I'm not sure if the bacteria were viable afterwards.
I use a Beckman J6-B with a JS 4.2 rotor (6 x 1 liter) to harvest bacteria used in protein expression. The rotor's maximum rcf is 3300 x g (average) and a half hour spin clarifies my supernatant.
First post here and not sure if I can post links, but if this shows up it's a good introduction to centrifugation principles:
Wiki User
∙ 11y ago
Wiki User
∙ 13y ago150 to 180 g force
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Function of crystal violet in gram stain?
Well if you stained with only crystal violet, then they would all be violet! If you do a Gram Stain the right way, you end up getting Gram positive being violet or purple and Gram Negative being red or pink.
What substrate amount is required to achieve the maximum reaction rate?
4.0 g of lactose substrate
What happened during g1 phase?
During G 1 phase, the cell grows in size and synthesizes mRNA and proteins that are required for DNA synthesis Cytoplasm is manufactured
What is the function of safranin in the Gram staining process?
Crystal violet is used first to stain all bacteria purple. However, when you immerse the bacteria then in alcohol, alcohol affects the permeability of the peptidoglycan layer to crystal violet, blocking its exit from gram-positive cells. This way, gram-positive cells remain purple while gram-negative cells are colourless. Safranin red is then used as a counterstain to make the two types of bacteria more differentiable.
What is the difference between red out and black out?
Redout is caused by negative G's (as in G forces) being induced on the body, causing blood to be forced up into one's head, and possibly causing them to lose consciousness. Blackout is almost the opposite: caused by positive G's, and resulting in blood being pulled away from one's head. Both, however, can result in a loss of consciousness. Both conditions are similar in the way they are generally only expierenced by military pilots conducting high G-force maneuvers.
Which law of motion creates the g force?
One way to think of the "g-force" is as a fictitious force - one that SEEMS to push you backwards, when you are being accelerated forward. Actually (from the point of view of an observer who is not accelerating with you), no backwards force is required - in fact, it is only by the fact that there is a forward force, NOT balanced by a backward force, that acceleration is possible in the first place.
What is g force in physics?
In physics g force means gravitation force
How do you play g-force in 3d?
play g force
When was G Force created?
G Force was created in 1982.
Who plays the ferret in g-force?
there is no ferret in g-force
Is there a difference between positive and negative g force?
Positive g-force is down. Negative g-force is up.
What does the G mean for G force?
The Movie? - Guinea Pig Force
What is the g force pilots experience?
what is the g force that pilots experience
What is a G-force?
gravitational force
What is a car's g-force?
There are accelerating G's, Braking G's and cornering G's. It is the force in that direction the car and occupants are exposed to. 1 G is the for of gravity.
Cheats for g-force the ds game?
How to destroy the camera in g force
Did Taylor swift star in g-force?
no, Taylor did not star in G-Force
