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Well if you stained with only crystal violet, then they would all be violet! If you do a Gram Stain the right way, you end up getting Gram positive being violet or purple and Gram Negative being red or pink.

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16y ago

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Is crystal violet considered a primary stain in the Gram staining technique?

Yes, crystal violet is considered a primary stain in the Gram staining technique.


What is the secondary stain in gram stain procedure?

It is crystal violet & stains all cells purple.


What function of iodine in gram stain?

fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.


What is the function of crystal violet in the gram staining process?

Crystal violet is used in the Gram staining process to initially stain all bacteria purple. This helps differentiate between Gram-positive and Gram-negative bacteria based on their ability to retain or lose the crystal violet dye during the staining process.


What is the primary stain used in Gram staining?

The primary stain used in Gram staining is crystal violet.


What is the purpose of crystal violet in the Gram's stain procedure?

Crystal violet is the primary stain in the Gram's stain procedure, used to color all bacteria cells purple. This helps differentiate between Gram-positive bacteria (which retain the violet color) and Gram-negative bacteria (which lose the violet color when decolorized with alcohol).


What is the purpose of the crystal violet in the gram stain procedure?

Its the primary stain of the procedure. IT stains the Gram positive organisms


What color will gram stain positive turn into?

If using a gram stain, they will turn Crystal Violet.


What is the function of iodine solution in the gram stain?

It allows the primary stain crystal violet to remain in the cell instead of being washed out. Due to the larger size of the crystal violet molecule, when the ethanol is applied (the decolorizer) the stain will not be washed out of the Gram (+) positive cells.


What are the reagents and steps min a Gram stain?

The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.


Function of acetone in gram staining?

Acetone is used for decolouring method, washing away the iodine-crystal violet complex formed in gram negative bacteria. so that the gram negative bacteria can be then stained with safranin or fuchsin.


What function of lugol's iodine in gram staining?

fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.