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What else can I help you with?
Why do you need to add primers DNA polymerase and nucleotides to your PCR mixture?
NEED OF PRIMER IN PCR-It is because the polymerase enzyme we use in the PCR only extend a DNA strand but not initiate its synthesis. So, to initiate the synthesis of DNA strand onto a template strand we require primers.
What is the use of primer in DNA replication?
DNA polymerase requires a primer because it can't initiate polymerization by it self only,but requires a preexisting free 3'OH group to which it can add deoxynucleotides forming phosphodiester bond & the free 3'OH group is provided by the primer.Therefore the DNA polymerase requires primer.
How she was able to add the HInd III restriction enzyme site in this position based on the result of her gel?
She was able to do so because the band pattern portrayed on the agarose gel shows exactly where the sequence AAGCTT was located on the DNA. Because of this, she was able to place the Hind III restriction enzyme right at that sequence since it was given that the enzyme recognizes that specific sequence.
How can I efficiently design primers with restriction sites for my molecular biology experiments?
To efficiently design primers with restriction sites for molecular biology experiments, use online tools like Primer3 to select appropriate primer sequences and add desired restriction sites. Ensure the restriction sites are compatible with the chosen enzyme and consider factors like primer length, melting temperature, and GC content for optimal primer design.
If E. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure then will reaction be carried out in waterbath?
No, the reaction will not be carried out in a water bath. E. coli DNA polymerase requires higher temperatures to function optimally for PCR, typically around 72°C. Therefore, a thermal cycler with the ability to cycle through different temperatures is needed to perform PCR with E. coli DNA polymerase.
Why do you need to add primers DNA polymerase and nucleotides to your PCR mixture?
NEED OF PRIMER IN PCR-It is because the polymerase enzyme we use in the PCR only extend a DNA strand but not initiate its synthesis. So, to initiate the synthesis of DNA strand onto a template strand we require primers.
List the components needed to perform PCR?
Primers - to provide the double stranded section of DNA that the enzyme needs to attach to and to make sure that you amplify the section you're interested in. dNTPs - nucleotide building blocks to make your PCR product Taq polymerase - the enzyme that will drive the reaction DNA - your template and sample of interest Usually you will also add a buffer and possibly magnesium chloride (depending on whether it's already contained in your buffer or not). The buffer ensures the reaction happens in the correct conditions (pH and so on). The magnesium chloride supplies the Mg ions that Taq polymerase needs as a co-enzyme. You also need a thermal cycler to run your reaction.
What happens if you forget to add sand to masonry cement mix?
there will not be enough strength
What happens if you forget to add starch before putting the iodine in a beverage?
you will die
What reagents do you use in pcr?
in pcr technique we take original dna first heat it to separate to strands in thermocycler then add rna primer after the formation of about 10 sequences on both parental strands add dna polymerase to construct further
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How does Polymerase Chain Reaction work?
Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.
Did you forget to add your middle name on your flight booking?
Yes, did you forget to include your middle name on your flight reservation?
If you have a small gene that you wish replicated by pcr you add radioactively labeled nucleotides to the pcr machine after 3 replication cycles how many double-stranded DNA molecules do you have?
After 3 replication cycles in PCR, the number of double-stranded DNA molecules doubles each cycle. Therefore, after 3 cycles, you would have 8 double-stranded DNA molecules.
What happen if you forget to add crystal violet to a stain?
You don't get a counterstain.
What is the function of primers?
RNA primers are used to initiate the DNA replication at the template strand. DNA molecules require a free 3' OH, to which it could add the nucleotides. This free 3' OH is provided by the RNA primer. So prior to the synthesis of DNA a short fragment of RNA is synthesized that is later excised and filled with DNA molecules.
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