As magnification increases, the depth of focus decreases.
If the fine focus knob is turned backwards the depth of the specimen decreases. When the knob is turned forward the depth of the specimen increases.
If magnification increases ONLY, then resolving power does not increase. However, if the magnification increased while staying in focus (upgrading resolution and magnification with objective lense), shorter wavelengths are needed to stay in focus with increased magnification to yield the same high resolution as with previous objective lense, so this case, resolving power does increase.
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The specimen should be in clear focus
By increasing the magnification the focus is increasing on a particular area due to which the no of light rays which were before more on the entire slide is now decreased due to focusing (magnifying) hence the brightness decreases due to decreased in no of light rays present in that small area
If the fine focus knob is turned backwards the depth of the specimen decreases. When the knob is turned forward the depth of the specimen increases.
The depth of field is the part of a specimen that is in sharp focus; the depth of field decreases as the NA increases. The depth of focus on the other hand is the magnified image in focus on the film plane; depth of focus decreases as magnification increases. To learn more about microscopes and its uses visit the website in the link below.
Each depth will show different parts of whatever you are looking at on the microscope. The focus improves when using the right depth and magnification.
If magnification increases ONLY, then resolving power does not increase. However, if the magnification increased while staying in focus (upgrading resolution and magnification with objective lense), shorter wavelengths are needed to stay in focus with increased magnification to yield the same high resolution as with previous objective lense, so this case, resolving power does increase.
They start to get smaller
To bring a sharp focus.
it could be either coarse or fine knob. coarse knob is used to focus image at higher magnification, while fine knob is used to focus on lower magnification
All magnification
The names of the parts of the microscope that change the magnification is the coarse focus knob.
Couse focus knob
what is the maximum depth of a deep-focus earthquake
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