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Direct demonstration of V.cholerae is not recommended for general purposes. However, darting motility and its inhibition of specific antisera, especially after brief inoculation in broth, has proven valuable in experienced hands for presumptive diagnosis. The method is as follows: Prepare a hanging drop from alkaline peptone water which has been inoculated with faeces and incubated at 37oC for 4-6 hours. Examine it under high power dry lens of microscope. Darting motility characteristic of vibrios is suggestive of diagnosis.

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What is the value of a hanging-drop preparation?

A hanging-drop preparation is used in microbiology to observe the motility of microorganisms. By placing a drop of liquid culture on a coverslip inverted over a concave slide, the microorganisms are suspended in a hanging drop of liquid, allowing for easy observation under a microscope.


What is the Motion of pseudomonas fluorescens in hanging drop preparation?

In a hanging drop preparation, Pseudomonas fluorescens can exhibit motility by moving actively through the liquid medium using flagella. The movement is typically characterized by a swift and smooth motion, allowing the bacteria to explore their environment efficiently within the hanging drop.


Purpose of the hanging drop.?

The hanging drop method is used in microbiology to grow and observe bacterial or yeast cultures. It involves suspending a drop of liquid culture from a coverslip over a depression slide. This setup allows for the observation of microbial growth in a controlled environment.


Does the hanging-drop technique have any practical value?

Yes, the hanging-drop technique is commonly used in laboratories for observing live cells or microorganisms under a microscope. It allows for the visualization of cellular behaviors and interactions in a controlled environment, making it a valuable tool in studying cell biology, microbiology, and drug discovery.


Can the hanging drop slide be used to examine other microorganisms?

Yes, the hanging drop slide can be used to examine a variety of microorganisms, not just bacteria. It can also be used to observe fungi, algae, and other small organisms by placing a sample in the hanging drop and observing their motility or characteristics under a microscope.

Related Questions

What is the value of a hanging-drop preparation?

A hanging-drop preparation is used in microbiology to observe the motility of microorganisms. By placing a drop of liquid culture on a coverslip inverted over a concave slide, the microorganisms are suspended in a hanging drop of liquid, allowing for easy observation under a microscope.


What is the Motion of pseudomonas fluorescens in hanging drop preparation?

In a hanging drop preparation, Pseudomonas fluorescens can exhibit motility by moving actively through the liquid medium using flagella. The movement is typically characterized by a swift and smooth motion, allowing the bacteria to explore their environment efficiently within the hanging drop.


Aside from motility what facts learned from hanging drop preparation?

The hanging drop preparation can study the motility over a longer period of time, but it is more difficult to examine than a wet mount. Wet mount is preferable to a hanging drop slide when using phase contrast optics.


Would you expect Brownian motion to increase the longer you observe a hanging drop or wet mount preparation?

Just by the sheer fact that any vibration of any kind can be considered Brownian motion for a hanging drop test. the longer you observe your bacteria, the more chance there are of Brownian motion interfering with your motility test ( I assume you're talking about the motility hanging drop, right?). It is because of this fact that the hanging drop test is quickly falling out of favor for more secure tests for motility, the semi-solid media being one of them.


Would you expect Brownian motion to increase the longer you observe a hanging drop preparation?

Just by the sheer fact that any vibration of any kind can be considered Brownian motion for a hanging drop test. the longer you observe your bacteria, the more chance there are of Brownian motion interfering with your motility test ( I assume you're talking about the motility hanging drop, right?). It is because of this fact that the hanging drop test is quickly falling out of favor for more secure tests for motility, the semi-solid media being one of them.


Does hanging drop technique have any practical value?

hanging drop tecnique have practical value


Why is petroleum jelly used in hanging drop method?

Petroleum jelly is used in the hanging drop method to create a barrier that prevents the sample drop from spreading out too much and allows it to hang down under the coverslip. This setup provides a controlled environment for observing microorganisms or cells under a microscope.


What advantages does hanging drop preparation have over a wet mount preparation?

Hanging drop slide is more resistant to evaporation because of the concave depression of the slide placed over the cells along with the petroleum jelly prevents drying. Wet mounts can dry out fairly quickly.


What is the use of hanging drop?

Hanging drop preparations can be observed for a long time because they do not dry up very quickly. Thus, motility of an organisim can be observed.


Why isn't the oil immersion lens used in hanging drop procedure?

the hanging drop procedure uses a thicker slide, which could crack if the oil immersion lens is used. My bio professor worte: hanging drop slides asre too thick to use under oil.


Purpose of the hanging drop.?

The hanging drop method is used in microbiology to grow and observe bacterial or yeast cultures. It involves suspending a drop of liquid culture from a coverslip over a depression slide. This setup allows for the observation of microbial growth in a controlled environment.


What is the silver line in hanging drop method?

The sliver line that you see is actually an artifact and the edge of the drop.