0
A palindrome in the context of a restriction enzyme site refers to a sequence of DNA that reads the same forward and backward. Many restriction enzymes recognize and cut DNA at palindromic sequences. This characteristic allows the enzyme to bind symmetrically to both strands of DNA.
What else can I help you with?
Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?
The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.
What is the specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site?
The specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site, is 5'-GAATTC-3'.
What is the term that describes the sequence of DNA that a restriction enzyme finds and cuts?
Template Sequence
What is a DNA restriction site?
The restriction site is a sequence of DNA that is recognized by an endonuclease, or a protein that cuts DNA, as a site at which the DNA is to be cut. This cutting happens when restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.
Why does raising the temperature to 42 degrees stop the enzyme restriction?
Raising the temperature to 42 degrees can denature the enzyme, changing its shape and disrupting its active site. This can prevent the enzyme from functioning properly, halting the restriction process.
What is the restriction site of the restriction enzyme TaqI?
TaqI's restriction site is:TCGAAGCT
What is the restriction site of the restriction enzyme Hae III?
The restriction site of Hae III is GGCC. It cuts between the G and the C. This produces blunt ends.
What is the restriction site to restriction enzyme Hae III?
Hae III cuts at the site GGCC. It creates blunt ends - meaning a clean cut. This is found between the G and C.
Where in the DNA sequence does the restriction enzyme EcoR1 specifically cut?
The restriction enzyme EcoR1 specifically cuts the DNA sequence at the recognition site GAATTC.
What is the specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site?
The specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site, is 5'-GAATTC-3'.
What is the term that describes the sequence of DNA that a restriction enzyme finds and cuts?
Template Sequence
What is a DNA restriction site?
The restriction site is a sequence of DNA that is recognized by an endonuclease, or a protein that cuts DNA, as a site at which the DNA is to be cut. This cutting happens when restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.
Why does raising the temperature to 42 degrees stop the enzyme restriction?
Raising the temperature to 42 degrees can denature the enzyme, changing its shape and disrupting its active site. This can prevent the enzyme from functioning properly, halting the restriction process.
What determines how DNA will be cut by a restriction enzyme?
Restriction enzymes cut DNA at specific sites called restriction sites. These restriction sites are typically 6 - 8 nucleotides in length and have a defined set of nucleotide bases. For example, the restriction enzyme Eco R1 cuts at the site: AGGTTC. Therefore, if the target DNA contains the above sequence, Eco R1 is able to cut it within the restriction site. Hence, by looking into the target site and which restriction enzymes are being used, on can make an accurate estimate of where the target DNA will be cut
How does an enzyme's active site relates to its substrate?
Their Shapes Fit Snugly Together.
What is the best explanation for why a restriction enzyme does not cut the DNA of the cell that produces it?
Restriction enzymes are made by cells to protect their own DNA from being cut. These cells produce a modification enzyme that adds a methyl group to specific sites on their own DNA sequence, which prevents the restriction enzyme from cutting. This process is known as "methylation protection."
Why restriction enzyme cannot cut its own DNA?
Restriction enzymes are produced by bacteria to help destroy foreign, invading DNA, such as the DNA of bacteriophage (a virus that infects bacterial cells). Every restriction enzyme comes with a methylase enzyme, or more specifically, a DNA methyltransferase. The methylase enzyme methylates (adds a methyl group) to the restriction endonuclease site on the cell's own DNA, which protects the sites from the restriction enzyme so that it does not degrade its own DNA.
