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Coagulase is a protein produced by several organisms, namely Staphylococcus and in laboratories is used to distinguish different isolates.
Bound coagulase is part of the larger family of MSCRAMM.
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∙ 12y ago
Wiki User
∙ 15y agoCoagulase is an enzyme that is produced by Staphylococcus aureus and converts fibrinogen to fibrin.
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Why is it important to use fresh cultures in a coagulase test?
Coagulase is a protein enzyme. It is important to use fresh cultures in a coagulase test because coagulase expression is highest during the log phase of growth.
Are vacuoles membrane bound?
Vacuoles are bound by a single unit membrane called tonoplast.
What are the biochemical tests to confirm staphylococcus?
Streptococci are not differentiated by biochemical tests, but rather by hemolysis reaction (beta Strep or alpha Strep) and sero-group (as in Group E Strep).Further, streptococcus is also identified by thr following tests:1. Coagulase.2. Haemolysin (It lyses 4% RBC suspension)3. Fibrinolysin (It can dissolve a fibrin clot)4. Strp. Pneumoniae also gives a positive bile solubility test.5. It ferments Inulin (A sugar)
Do eukaryotic cells have membrane-bound organelles?
Eukaryotes contain membrane bound organelles. Prokaryotes do not contain membrane bound organelles. However, prokaryotes do contain ribosomes which is an example of a non-membrane bound organelle.
What do eukaryotic Cells have that prokaryotic Cells do not have?
nucleus & other membrane-bound organelles.
What is difference between tube coagulase and slide coagulase test?
tube coagulase is done in test tube while slide coagulase is done on slide.thnk you all! cheeres!
Why is it important to use fresh cultures in a coagulase test?
Coagulase is a protein enzyme. It is important to use fresh cultures in a coagulase test because coagulase expression is highest during the log phase of growth.
What are 2 types of staphylococal coagulase?
2 types of staphylococal coagulase are 1.staph.epidermidis/albus2.staph.aureus
What is a free coagulase and its significance?
Free coagulase is an extracellular toxin that reacts with a thrombin-like, thermostable substance known as coagulase-reacting factor (CRF) or serum factor. The reaction of coagulase and CRF will lead to the formation of coagulase-CRF complex where this complex indirectly changes frbrinogen to fibrin (clotting factor) and hence will form a fibrin clot. Furthermore, it is also use as the primary indicator of virulence among staphylococci. Its clinical significance is that when doing the coagulase tube test, the free coagulase will react with the plasma in the tube and hence convert the fribrinogen into fibrin which lead to fibrin clot formation in the tube. This is shown after one day of incubation of the tube, when the tube being invert, the plasma will not go down showing that it is clotted.
What is a CNS and what do they do?
coagulase-negative staphylococci (CNS)
Is coagulase test a microbiological test?
YES
What is the carbohydrate profile for S epidermidis?
coagulase
What makes coagulase important for a pathogen?
There is some controversy about whether coagulase is a virulence factor, but one way coagulase contributes to pathogenicity is that it binds prothrombin to form staphylothrombin, which then cleaves fibrinogen to form fibrin clots in which the bacteria can live and avoid phagocytosis by neutrophils.
What does the enzyme coagulase do?
The coagulase enzyme activates prothrombin, which causes fibrin formation around the bacteria (ie Staph aureus), protecting it from phagocytosis. Of all 3 pathogenic staphylococcal species, only Staph aureus is coagulase positive.
What is a coagulase test used for?
Rabbit plasma, sometimes with EDTA added
Micrococcus luteus produces the enzyme coagulase?
Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, spherical, saprotrophic bacterium that belongs to the family Micrococcaceae. It produces coagulase which is a protein enzyme that enables the conversion of fibrinogen to fibrin.
What is the principle behind the coagulase test?
in the coagulase test, normally a virulent staphylococcus is put into plasma. plasma contains the plasma protein fibrinogen. when fibrinogen is acted upon by a fibrinogenase, it is then converted to fibrin. fibrin has te ability to form clots. so in the case of staphylococcus aureus which has the enzyme coagulase also called fibrinogenase, the enzyme acts on the fibrinogen in plasma which is converted to fibrin hence the clots that are seen suspending in the solution after 4 hours.
