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What is isozymes?

Updated: 4/28/2022

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CA isozymes

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Why the LDH isoenzymes can be separated by electrophoresis?

In the name of ALLAh , In order to answer this qustation Why the LDH isoenzymes can be separated by electrophoresis? .... we are going to answer this questions……….. 1) WHAT is ELECTROPHORESIS ? 2) BIOCHEMICAL COMPOSATION of LDH ? First .. I will introduce information about ELECTROPHORESIS :- What is Electrophoresis ? Electophoresis is aprocess of separating substance according to their electric charge which they carrying by introducing this substances in electrical media whish carrying out -ve and +ve poles . Electrophoresis is an analytical method frequently used in molecular biology and medicine. It is applied for the separation and characterization of proteins, nucleic acids and subcellular-sized particles like viruses and small organelles. Its principle is that the charged particles of a sample migrate in an applied electrical field. If conducted in solution, samples are separated according to their surface net charge density. The most frequent applications, however, use gels (polyacrylamide, agarose) as a support medium. The presence of such a matrix adds a sieving effect so that particles can be characterized by both charge and size. Protein electrophoresis is often performed in the presence of a charged detergent like sodium dodecyl sulfate (SDS) which usually equalizes the surface charge and, therefore, allows for the determination of protein sizes on a single gel. Additives are not necessary for nucleic acids which have a similar surface charge irrespective of their size BIOCHEMISTERY of LDH :- Lactate dehydrogenase (LDH) catalyzes the reaction below. This reaction provides an important source of NAD+ for cells undergoing anaerobic glycolysis. Pyruvate + NADH + H+ Lactate + NAD+ LDH is a tetrameric protein consisting of two types of subunits, called M and H, which have small differences in amino acid sequence. Different molecular forms of an enzyme are called isoenzymes or isozymes. M subunits predominate in skeletal muscle and liver, and H subunits predominate in heart. M and H subunits combine randomly with each other, so that the five major isoenzymes have the compositions M4, M3H, M2H2, MH3, and H4. Because of random subunit reassortment, the isoenzymic composition of a tissue is determined primarily by the activities of the genes specifying the two subunits. This propartey can indicate why LDH has electrophorises activity. ABD ELRHMAN OSAMA , abdelrhmanosama@yahoo.com , 4th year faculty of medicine , Al-azher univ., EGYPT .

Related questions

How do you spell isoeymes?

The word is spelled Isozymes (also known as isoenzymes). These are enzymes that have different sequences of amino acids with different properties. Isozymes were discovered in 1957 by Hunter and Markert who noted that these enzymes catalyzed the same chemical reaction and had identical functions in the same individual. Isozymes as variants of the original can change a gene structure so that as one function of the enzyme flourishes, the other can become extinct.

What is Glutathion-S-Transferases in liver fluke?

Glutathion-S-Transferases are a group of isozymes i.e.,they are a group of aminoacids,that catalyse a particular reaction.They act as detoxifying agents on xenobiotics.Xenobiotics are nothing but drugs that are used to act upon some animal or organism.Xenobiotics are not naturally produced in body.So this Glutathion-S-Transferases,a group of isozymes in liver fluke,protect the organism from drugs and antibiotics.

What are some eight letter words with 1st letter I and 3rd letter O and 4th letter Z and 5th letter Y and 8th letter S?

According to SOWPODS (the combination of Scrabble dictionaries used around the world) there are 1 words with the pattern I-OZY--S. That is, eight letter words with 1st letter I and 3rd letter O and 4th letter Z and 5th letter Y and 8th letter S. In alphabetical order, they are: isozymes

What are some eight letter words with 2nd letter S and 3rd letter O and 4th letter Z?

According to SOWPODS (the combination of Scrabble dictionaries used around the world) there are 2 words with the pattern -SOZ----. That is, eight letter words with 2nd letter S and 3rd letter O and 4th letter Z. In alphabetical order, they are: isozymes isozymic

What are some eight letter words with 1st letter I and 5th letter Y and 8th letter S?

According to SOWPODS (the combination of Scrabble dictionaries used around the world) there are 4 words with the pattern I---Y--S. That is, eight letter words with 1st letter I and 5th letter Y and 8th letter S. In alphabetical order, they are: inlayers isohyets isotypes isozymes

What are some eight letter words with 1st letter I and 3rd letter O and 5th letter Y?

According to SOWPODS (the combination of Scrabble dictionaries used around the world) there are 5 words with the pattern I-O-Y---. That is, eight letter words with 1st letter I and 3rd letter O and 5th letter Y. In alphabetical order, they are: isohyets isotypes isotypic isozymes isozymic

What are some eight letter words with 1st letter I and 2nd letter S and 5th letter Y and 6th letter M?

According to SOWPODS (the combination of Scrabble dictionaries used around the world) there are 2 words with the pattern IS--YM--. That is, eight letter words with 1st letter I and 2nd letter S and 5th letter Y and 6th letter M. In alphabetical order, they are: isozymes isozymic

What are some eight letter words with 2nd letter S and 3rd letter O and 4th letter Z and 5th letter Y?

According to SOWPODS (the combination of Scrabble dictionaries used around the world) there are 2 words with the pattern -SOZY---. That is, eight letter words with 2nd letter S and 3rd letter O and 4th letter Z and 5th letter Y. In alphabetical order, they are: isozymes isozymic

What are some eight letter words with 5th letter Y and 6th letter M and 7th letter E and 8th letter S?

According to SOWPODS (the combination of Scrabble dictionaries used around the world) there are 2 words with the pattern ----YMES. That is, eight letter words with 5th letter Y and 6th letter M and 7th letter E and 8th letter S. In alphabetical order, they are: berhymes isozymes

What are some eight letter words with 5th letter Y and 6th letter M and 8th letter S?

According to SOWPODS (the combination of Scrabble dictionaries used around the world) there are 7 words with the pattern ----YM-S. That is, eight letter words with 5th letter Y and 6th letter M and 8th letter S. In alphabetical order, they are: anonymas berhymes comsymps ecthymas euonymus evonymus isozymes

What are some eight letter words with 3rd letter O and 4th letter Z and 7th letter E and 8th letter S?

According to SOWPODS (the combination of Scrabble dictionaries used around the world) there are 2 words with the pattern --OZ--ES. That is, eight letter words with 3rd letter O and 4th letter Z and 7th letter E and 8th letter S. In alphabetical order, they are: isozymes swozzles

What are some eight letter words with 2nd letter S and 6th letter M and 7th letter E and 8th letter S?

According to SOWPODS (the combination of Scrabble dictionaries used around the world) there are 2 words with the pattern -S---MES. That is, eight letter words with 2nd letter S and 6th letter M and 7th letter E and 8th letter S. In alphabetical order, they are: isonomes isozymes

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