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In the name of ALLAh , In order to answer this qustation Why the LDH isoenzymes can be separated by electrophoresis? .... we are going to answer this questions……….. 1) WHAT is ELECTROPHORESIS ? 2) BIOCHEMICAL COMPOSATION of LDH ? First .. I will introduce information about ELECTROPHORESIS :- What is Electrophoresis ? Electophoresis is aprocess of separating substance according to their electric charge which they carrying by introducing this substances in electrical media whish carrying out -ve and +ve poles . Electrophoresis is an analytical method frequently used in molecular Biology and medicine. It is applied for the separation and characterization of proteins, nucleic acids and subcellular-sized particles like viruses and small organelles. Its principle is that the charged particles of a sample migrate in an applied electrical field. If conducted in solution, samples are separated according to their surface net charge density. The most frequent applications, however, use gels (polyacrylamide, agarose) as a support medium. The presence of such a matrix adds a sieving effect so that particles can be characterized by both charge and size. Protein electrophoresis is often performed in the presence of a charged detergent like sodium dodecyl sulfate (SDS) which usually equalizes the surface charge and, therefore, allows for the determination of protein sizes on a single gel. Additives are not necessary for nucleic acids which have a similar surface charge irrespective of their size BIOCHEMISTERY of LDH :- Lactate dehydrogenase (LDH) catalyzes the reaction below. This reaction provides an important source of NAD+ for cells undergoing anaerobic glycolysis. Pyruvate + NADH + H+ <=> Lactate + NAD+ LDH is a tetrameric protein consisting of two types of subunits, called M and H, which have small differences in amino acid sequence. Different molecular forms of an enzyme are called isoenzymes or isozymes. M subunits predominate in skeletal muscle and liver, and H subunits predominate in heart. M and H subunits combine randomly with each other, so that the five major isoenzymes have the compositions M4, M3H, M2H2, MH3, and H4. Because of random subunit reassortment, the isoenzymic composition of a tissue is determined primarily by the activities of the genes specifying the two subunits. This propartey can indicate why LDH has electrophorises activity. ABD ELRHMAN OSAMA , abdelrhmanosama@Yahoo.com , 4th year faculty of medicine , Al-azher univ., Egypt .
What else can I help you with?
What is the function of comb in electrophoresis?
The comb is used to create wells in the gel where samples can be loaded for electrophoresis. It helps to organize the samples and ensure that they are separated properly during the process.
What are bands in electrophoresis?
Bands in electrophoresis refer to the distinct areas of separated molecules on a gel, appearing as lines or streaks. Each band represents a different size or charge of the molecules being separated, allowing for analysis and quantification in biochemistry and molecular biology studies. Detection of bands can be achieved through staining or fluorescence techniques after gel electrophoresis.
What is the principle for agarose gel electrophoresis?
Agarose gel electrophoresis is based on the principle that DNA molecules are negatively charged and will migrate towards the positive electrode in an electric field. The smaller DNA fragments move faster through the agarose gel matrix, allowing for separation based on size. UV light is commonly used to visualize the separated DNA bands after electrophoresis.
How can RNA be separated and visualized using acrylamide gel electrophoresis?
RNA can be separated and visualized using acrylamide gel electrophoresis by first denaturing the RNA samples, then loading them onto the gel and applying an electric current. The RNA molecules will move through the gel based on their size, with smaller molecules moving faster. After electrophoresis, the gel can be stained with a dye that binds to RNA, allowing the bands to be visualized under UV light.
Difference between southern and northern blotting?
Southern Blotting refers to the identification of detailed sequences of DNA in which the DNA fragments are separated by electrophoresisNorthern Blotting refers to the identification of detailed sequences of RNA in which the RNA fragments are separated by electrophoresis
How are DNA fragments separated on DNA fingerprinting?
Gel electrophoresis
How many ions does electrophoresis have?
Electrophoresis is a process by which molecules are separated based on band length. That said, your question makes no sense.
What is the name of the technique commonly used to separate different isoenzymes from one another?
The technique commonly used to separate different isoenzymes from one another is called gel electrophoresis. This method takes advantage of each isoenzyme's unique electrophoretic mobility in a gel matrix to separate them based on their size and charge differences.
What is the function of comb in electrophoresis?
The comb is used to create wells in the gel where samples can be loaded for electrophoresis. It helps to organize the samples and ensure that they are separated properly during the process.
What are bands in electrophoresis?
Bands in electrophoresis refer to the distinct areas of separated molecules on a gel, appearing as lines or streaks. Each band represents a different size or charge of the molecules being separated, allowing for analysis and quantification in biochemistry and molecular biology studies. Detection of bands can be achieved through staining or fluorescence techniques after gel electrophoresis.
Process that restriction fragments of DNA are separated from each other by the use of electricity?
The process you are referring to is called electrophoresis. In this technique, DNA fragments are loaded onto a gel matrix and an electric current is applied. The negatively charged DNA molecules move towards the positive electrode, separating based on size and charge.
Cause of high ldh?
very much so.
An analysis of isoenzymes in blood serum can be used to?
Isoenzymes in blood serum can be used to diagnose disease.
What color is the tube for CPK isoenzymes?
The tube for CPK (creatine phosphokinase) isoenzymes is typically red or gold, depending on the laboratory.
What are the isoenzymes of phosphatidylinositol synthase?
psi1
What is the principle for agarose gel electrophoresis?
Agarose gel electrophoresis is based on the principle that DNA molecules are negatively charged and will migrate towards the positive electrode in an electric field. The smaller DNA fragments move faster through the agarose gel matrix, allowing for separation based on size. UV light is commonly used to visualize the separated DNA bands after electrophoresis.
How can RNA be separated and visualized using acrylamide gel electrophoresis?
RNA can be separated and visualized using acrylamide gel electrophoresis by first denaturing the RNA samples, then loading them onto the gel and applying an electric current. The RNA molecules will move through the gel based on their size, with smaller molecules moving faster. After electrophoresis, the gel can be stained with a dye that binds to RNA, allowing the bands to be visualized under UV light.
