What is streak dilution plate technique?

Answer 1

"dilute" is not really the right word for it but... A proper streaking method (4 streaks total recommended but some hospital policy prefer 3) can separate and isolate the clinically significant bacteria (the one who makes the patient sick). With each streak, the used metal loop must be incinerated or a used plastic loop should be thrown away AND the streaking lines should have space to each other(except the 1st streak) to ensure the none of the flora are being isolated or else getting the correct results will be difficult.

Answer 2

The streak plate technique is a method of diluting bacteria down suficiently so that the will grow as single colonies. The technique varies from individual to individual so much so that you can identify a researcher's plates much like their handwritting! The technique is somewhat more standardised in hospital labs and a printed out sheet is placed below the plate for the operative to follow as a guide. The technique is usually taught like this; 1) Flame your loop and aseptically take 1 loopful of culture and place it a 12 o'clock on your plate draw a straight line 5cm across the plate ending around 2.30o'clock. 2) Lift the loop and draw two more lines parallel the first about 0.5 cm distance below the first. 3) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines over lapping the first set. (your end at 5o'clock) 4) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the second set. (you end at 6.30o'clock) 5)Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the third set. (your end at 8o'clock) 6) Flame your loop this time instead of a set of lines start by overlapping the fourth set of lines and then draw a scribble into the middle of your plate using as much of the unused agar as possible. The technique is sort of a dilution becasue each time you flame your loop it is sterilised, when you then draw out some of the bacteria from your last set of lines and spread them over a much greater area.

Answer 3

Streak dilution plate technique is a method used to isolate individual bacterial colonies from a mixed culture. A small amount of the mixed culture is streaked onto the surface of an agar plate using a sterile loop, creating a pattern of decreasing concentration. This allows for the separation and growth of individual colonies for further analysis.