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Could some bacteria grow on the streak plate and not be seen using the pour plate technique?
Wiki User
∙ 9y ago
Put simply - yes. Some strictly aerobic organisms will not grow in a pour plate. They may, however proliferate on a streak plate. Also consider the posibility of experimental error. The culture may have been added to the molten agar when it was too hot for the organisms to survive.
Wiki User
∙ 14y ago
Wiki User
∙ 9y agoYes, although aerobic bacteria will not grow when using the pour plate technique, it can grow on the streak plate. Bacteria that is sensitive to low oxygen may not grow on a pour plate.
Wiki User
∙ 9y agoYes. Some aerobic organisms do not grow in a pour plate but they can proliferate on a streak plate. Experminental errors may also lead to bacterial growth.
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What happens if you rub the mineral across a streak plate but the mineral does not leave a streak?
The lack of a streak would indicate that the mineral is harder than the streak plate, or the color of the streak is the same as the color of the streak plate.
Advantages of pour plate method over other methods of bacterial colony?
The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.
What is meant by streak dilution technique?
The streak plate technique is a method of diluting bacteria down suficiently so that the will grow as single colonies. The technique varies from individual to individual so much so that you can identify a researcher's plates much like their handwritting! The technique is somewhat more standardised in hospital labs and a printed out sheet is placed below the plate for the operative to follow as a guide. The technique is usually taught like this; 1) Flame your loop and aseptically take 1 loopful of culture and place it a 12 o'clock on your plate draw a straight line 5cm across the plate ending around 2.30o'clock. 2) Lift the loop and draw two more lines parallel the first about 0.5 cm distance below the first. 3) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines over lapping the first set. (your end at 5o'clock) 4) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the second set. (you end at 6.30o'clock) 5)Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the third set. (your end at 8o'clock) 6) Flame your loop this time instead of a set of lines start by overlapping the fourth set of lines and then draw a scribble into the middle of your plate using as much of the unused agar as possible. The technique is sort of a dilution becasue each time you flame your loop it is sterilised, when you then draw out some of the bacteria from your last set of lines and spread them over a much greater area.
The calor a mineral leaves when it is rubbed across a plate?
Streak is the color of the finely powdered mineral when rubbed across a plate. Streak is one of the physical properties of minerals used to identify which specific mineral it is. Some minerals leave a completely different color streak than the original color of the whole mineral.
Compare the pour plate and streak plate methods of isolating bacteria?
I got the answer but you hav ta do your labreport yourself..haha Whoever wrote this is an a$$hole. I wish that you went to my school so I could kick your aXX..Ha ha back at you, jerk! thanks jerk,,that was really helpful.. anyway guys, here is the correct and precise answer, hope it will help u to complete ur lab repor..:) In streak plate method, After the first sector is streaked in dish, the inoculating loop is sterilized and an inoculum for the second sector is obtained from the first sector. The same is done for third and fourth sector. Thus this is a dilution process. Eventually, very few cells will be on inoculating loop, a single cell will drop from it as it is rubbed along the agar surface. These develop into separate colonies. Whereas, in the pour plate culture, sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating. After the agar has hardened each cell is fixed in place and forms an individual colony.
What is the simplest technique for isolating bacteria in growth media is referred to as the?
Streak-plate method
What is the purpose of the streak for isolation?
By using streak plate technique to spread a clinical sample out on the surface of a growth medium individual types of bacteria can be isolated
What is the differences between a streak plate technique and a serial dilution technique?
what is serial dilution and spread plate technique
What will a streak plate with two species of bacteria look like?
A streak plate, with 2 species of bacteria, will show the bacteria in straight lines. Each species of bacteria will be separate from the other.
Will diamond leave a streak on a streak plate?
Diamond will not leave a streak on a porcelain streak plate because diamond is harder than the streak plate. It will leave a scratch on the streak plate for the same reason.
Why use a streak plate to grow a bacterium?
You do a streak plate in order to get isolated colonies. If you inoculate into a slant, you have less surface area to work and less chance of getting isolated colonies. In broth, you'll definitely get growth but you won't know WHAT is growing. You go back into each quadrant (a little) with your loop in order to "dilute" the bacteria and get colonies. Quadrant 1 is pretty think (like a smear on the plate) but by the time you get to Quadrants 3 and 4, you should see more defined colonies and not just a film of bacteria.
Primary purpose of the streak plate?
A streak plate technique is used to isolate a single species from a mixed species population. You take a small sample of the mixed species on a sterile loop and streak an agar medium into four zones, reflaming the loop between zones. After incubation, single species colonies should be visible within the fourth zone.
When would a microbiologist want to use the streak-to-grow method.?
Plate streaking is often done to isolate a colony of bacteria. For instance, if a broth was grown with 2 or 3 different types of bacteria, it could be streaked out in a "3-phase streak." In a 3-phase streak the initial streak takes up a very small area (we draw a T on the back of the plate, this streak goes in the section above the T). The loop is flamed to kill off any bacteria still on it, then a couple streaks are made out of the original. This grabs some of the bacteria from that concentrated streak and spreads them out. This is repeated once more until the final streaks are less and less concentrated bacteria. When we incubate the plate we'll find lots of growth where the original streak was and less and less as we follow the path of the 3-phases. What you're looking for now is a single colony, off by itself. This can then be scraped off and plated on a separate plate and considered a "pure colony." Another usual time to use streak-to-grow bacteria is when you want to know the quantity or concentration of bacteria. You take maybe 0.1mL of solution and plate it, then count the number of colonies that form. Say you have 56 colonies, now you can say: 56 cfu (colony forming units) -------- 0.1 mL or 560 cfu/mL This is usual when testing to see whether a sterilization technique worked, or if a product is within the regulated levels of bacteria to be released the public, etc etc.
When would a microbiologist want to use the streak-to-grow method?
Plate streaking is often done to isolate a colony of bacteria. For instance, if a broth was grown with 2 or 3 different types of bacteria, it could be streaked out in a "3-phase streak." In a 3-phase streak the initial streak takes up a very small area (we draw a T on the back of the plate, this streak goes in the section above the T). The loop is flamed to kill off any bacteria still on it, then a couple streaks are made out of the original. This grabs some of the bacteria from that concentrated streak and spreads them out. This is repeated once more until the final streaks are less and less concentrated bacteria. When we incubate the plate we'll find lots of growth where the original streak was and less and less as we follow the path of the 3-phases. What you're looking for now is a single colony, off by itself. This can then be scraped off and plated on a separate plate and considered a "pure colony." Another usual time to use streak-to-grow bacteria is when you want to know the quantity or concentration of bacteria. You take maybe 0.1mL of solution and plate it, then count the number of colonies that form. Say you have 56 colonies, now you can say: 56 cfu (colony forming units) -------- 0.1 mL or 560 cfu/mL This is usual when testing to see whether a sterilization technique worked, or if a product is within the regulated levels of bacteria to be released the public, etc etc.
How do you isolate the bacteria from mixed culture which contains Fungus?
carefully pick up a small colony of bacteria from the plate with an inoculation loop and streak out a fresh medium containing plate
What advantage does the streak plate method have over the pour plate method?
A disadvantage of the streak plate technique could be colony isolation problems. If the streaking technique is not done properly or if there is too much of an organism present on the inoculating loop then the cells can cluster and form what looks like one colony but it can actually be a couple colonies (made from a couple cells). This can cause an inaccurate colony forming unit count.
The important of obtain pure bacteria?
The need of pure culture of bacteria to characterize an individual species. Pure culture are also important to study the morphology and physiology of individual bacterial species, their biochemical behaviour and response to different compounds like antibiotics, which all can me alter by the influence of other species if prestent (in mixed culture) and also for isolating and studying of their molicular structure i.e. DNA or RNA. Some common ways to obtain a pure culture of bacteria are: 1 The spread plate technique. 2 The pour plate. 3 Streak plate technique.
