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The streak plate technique is a method of diluting bacteria down suficiently so that the will grow as single colonies. The technique varies from individual to individual so much so that you can identify a researcher's plates much like their handwritting! The technique is somewhat more standardised in hospital labs and a printed out sheet is placed below the plate for the operative to follow as a guide. The technique is usually taught like this;
1) Flame your loop and aseptically take 1 loopful of culture and place it a 12 o'clock on your plate draw a straight line 5cm across the plate ending around 2.30o'clock.
2) Lift the loop and draw two more lines parallel the first about 0.5 cm distance below the first.
3) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines over lapping the first set. (your end at 5o'clock)
4) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the second set. (you end at 6.30o'clock)
5)Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the third set. (your end at 8o'clock)
6) Flame your loop this time instead of a set of lines start by overlapping the fourth set of lines and then draw a scribble into the middle of your plate using as much of the unused agar as possible.
The technique is sort of a dilution becasue each time you flame your loop it is sterilised, when you then draw out some of the bacteria from your last set of lines and spread them over a much greater area.
What else can I help you with?
What happens if you rub the mineral across a streak plate but the mineral does not leave a streak?
The lack of a streak would indicate that the mineral is harder than the streak plate, or the color of the streak is the same as the color of the streak plate.
Could some bacteria grow on the streak plate and not be seen using the pour plate technique?
Put simply - yes. Some strictly aerobic organisms will not grow in a pour plate. They may, however proliferate on a streak plate. Also consider the posibility of experimental error. The culture may have been added to the molten agar when it was too hot for the organisms to survive.
What is the gradual mixing of substances called?
dilution
What is a streak when it comes to talking about science?
'streak' is the color that a mineral will leave when rubbed on a piece of unglazed porcelain. It is used to help identify the mineral. For example, arsenopyrite looks very similar to gold, but has a dark grey or black streak. Gold has a yellow streak.
Colors of a line of fine powder from a mineral?
It is called the streak, and refers to the color of the powdered mineral that is left on a ceramic streak plate after the mineral specimen has been drawn across it. It may be different than the color of the observed specimen and is representative of the true color of a mineral that does not include impurities or traces of other minerals, or has been irradiated or heated.
What is the differences between a streak plate technique and a serial dilution technique?
A streak plate technique is used to isolate individual bacterial colonies on a solid agar plate to obtain pure cultures, while a serial dilution technique is used to dilute a bacterial sample in a series of steps to obtain a range of concentrations for further analysis. Streak plate technique is qualitative, focusing on colony isolation, while serial dilution technique is quantitative, focusing on estimating bacterial concentration.
Why the technique is called a four-way streak?
because you streak four times
Why peptone water is used in the dilution technique?
Peptone water is used in the dilution technique because it provides a nutrient-rich environment that supports the growth of bacteria. This allows for easier detection and enumeration of bacteria present in samples. Peptone water also helps maintain bacterial viability during dilution procedures.
What is the type of plating technique that is probably performed when using the above dilution technique?
The plating technique most likely performed when using the dilution technique is spread plating. In spread plating, a sample is spread over the surface of the agar plate using a sterile spreading tool to obtain individual colonies. This method helps to isolate and quantify bacteria present in the sample.
What is helium dilution technique?
Helium dilution technique is a method used for measuring lung volumes such as total lung capacity and residual volume. It involves the subject breathing a known volume of helium gas from a spirometer, which then mixes with the gas in the lungs. By analyzing the change in helium concentration, lung volumes can be calculated.
Why is the streak plate preferred over the spot inoculations in this procedure?
The streak plate method is preferred over spot inoculations because it allows for the isolation of individual colonies from a mixed culture, promoting the separation of different microorganisms. This technique creates a gradient of dilution across the agar plate, enabling the growth of distinct colonies that can be easily identified and characterized. Additionally, the streak plate method minimizes the risk of contamination and provides a more systematic approach to isolating pure cultures.
Common mistake in dilution streak?
One common mistake in dilution streaking is applying too much pressure on the loop while streaking, resulting in overlapping of bacteria and inaccurate dilution. This can lead to incorrect colony counts and difficulty in isolating single colonies. It is important to maintain a light touch on the agar surface to achieve proper dilution and clear separation of colonies.
What is the purpose of the streak for isolation?
By using streak plate technique to spread a clinical sample out on the surface of a growth medium individual types of bacteria can be isolated
What is the technique you used in purify the bacterial culture on a plate?
I used streak plate technique to purify the bacterial culture on a plate. This involved streaking the culture onto the agar surface in a specific pattern to isolate individual colonies by dilution. Subsequent incubation allowed the colonies to grow separately, enabling the selection of pure cultures for further study.
What is the hardness of stellite in rockwell?
In depends on the hardfacing technique and the final Fe dilution. Typical values range 42-45 HRC.
What are some advantages and disadvantages of the serial dilution agar plate technique?
the total count includes dead as well as living cells
What is the simplest technique for isolating bacteria in growth media is referred to as the?
The simplest technique for isolating bacteria in growth media is referred to as streak plating. In streak plating, a small sample containing mixed bacterial populations is spread in a pattern over the surface of an agar plate, allowing individual bacterial colonies to form and grow separately.
