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The streak plate technique is a method of diluting bacteria down suficiently so that the will grow as single colonies. The technique varies from individual to individual so much so that you can identify a researcher's plates much like their handwritting! The technique is somewhat more standardised in hospital labs and a printed out sheet is placed below the plate for the operative to follow as a guide. The technique is usually taught like this;
1) Flame your loop and aseptically take 1 loopful of culture and place it a 12 o'clock on your plate draw a straight line 5cm across the plate ending around 2.30o'clock.
2) Lift the loop and draw two more lines parallel the first about 0.5 cm distance below the first.
3) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines over lapping the first set. (your end at 5o'clock)
4) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the second set. (you end at 6.30o'clock)
5)Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the third set. (your end at 8o'clock)
6) Flame your loop this time instead of a set of lines start by overlapping the fourth set of lines and then draw a scribble into the middle of your plate using as much of the unused agar as possible.
The technique is sort of a dilution becasue each time you flame your loop it is sterilised, when you then draw out some of the bacteria from your last set of lines and spread them over a much greater area.
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Could some bacteria grow on the streak plate and not be seen using the pour plate technique?
Put simply - yes. Some strictly aerobic organisms will not grow in a pour plate. They may, however proliferate on a streak plate. Also consider the posibility of experimental error. The culture may have been added to the molten agar when it was too hot for the organisms to survive.
What happens if you rub the mineral across a streak plate but the mineral does not leave a streak?
The lack of a streak would indicate that the mineral is harder than the streak plate, or the color of the streak is the same as the color of the streak plate.
What is the gradual mixing of substances called?
dilution
What is a streak when it comes to talking about science?
'streak' is the color that a mineral will leave when rubbed on a piece of unglazed porcelain. It is used to help identify the mineral. For example, arsenopyrite looks very similar to gold, but has a dark grey or black streak. Gold has a yellow streak.
Colors of a line of fine powder from a mineral?
It is called the streak, and refers to the color of the powdered mineral that is left on a ceramic streak plate after the mineral specimen has been drawn across it. It may be different than the color of the observed specimen and is representative of the true color of a mineral that does not include impurities or traces of other minerals, or has been irradiated or heated.
What is the differences between a streak plate technique and a serial dilution technique?
what is serial dilution and spread plate technique
Common mistake in dilution streak?
ccc
What is helium dilution technique?
How is FRC measured by helium dilution, in specific detail.
Why the technique is called a four-way streak?
because you streak four times
What test is used to further identify unknown gram negative?
Serial dilution technique
Why are agar plates dried before being used for the preparation of dilution streak plates?
perhaps it is easier to streak that way, i mean when the agar is set and dry. .
Why must have 4 part in streaking technique?
so that you can get isolated colonies in the last streak . . . As you streak contineously you inoculum quantity decreases . . there by when you reach the end of last streak you get separate and isolated colonies . .
What is the purpose of the streak for isolation?
By using streak plate technique to spread a clinical sample out on the surface of a growth medium individual types of bacteria can be isolated
What is the simplest technique for isolating bacteria in growth media is referred to as the?
Streak-plate method
What is the hardness of stellite in rockwell?
In depends on the hardfacing technique and the final Fe dilution. Typical values range 42-45 HRC.
What are some advantages and disadvantages of the serial dilution agar plate technique?
the total count includes dead as well as living cells
Why is the streak-stab technique preferred rather than incubating the plates anaerobically?
The streak-stab technique is preferred over incubating the plates anaerobically because when isolating colonies allows biochemical testing to be performed. When the plate is incubated anaerobically it lacks oxygen and can not be biochemically tested.
