0
Phenylethyl Alcohol Agar (PEA) is prepared using the following incredients:
- Pancreatic Enzyme digest of Casein.......15.0g
- Papaic digest of Soybean Meal.................5.0g
- Sodium Chloride.....................................5.0g
- Yeast Extract..........................................5.0g
- Phenylethanol........................................2.5g
- Vitamin K..........................................10.0mg
- Hemin.................................................5.0mg
- L-Cystine.............................................0.4mg
- Sheep Blood........................................50.0ml
- Agar.....................................................15.0g
Components are mixed in 1 L water. If making less than 1 L media, change components accordingly. Media should be prepared at pH 7.3 +/- 0.2. After mixing components and adjusting pH, autoclave at 121 degrees C for 20 min then placed into water bath at 50 degrees C to allow it to cool enough to be poured.
Wiki User
∙ 14y ago
Add your answer:
Earn +20 pts
Which ingredient in PEA supplies carbon?
Casein is the ingredient in PEA that supplies carbon, as well as nitrogen. PEA is also known as Phenylethyl Alcohol Blood Agar.
What are the ingredients in PEA that supply carbon?
There are many ingredients in PEA that supply carbon. Some of the ingredients are dextrose, yeast extract, animal tissue, and soy peptone.
How might pea agar inhibit both gram negative and gram positive?
By containing more than 25% phenylethyl alcohol.
What is phenyl ethyl alcohol agar?
Phenylethyl alcohol agar (PEA) is a selective media used to cultivate Gram (+)microorganisms. The active ingredient, phenylethyl alcohol, inhibits or reduces growth of Gram (-) microorganisms by interfering with DNA synthesis.
What is the composition of a minimal salt with glucose agar?
Glucose 5.0g Ammonium phosphate monobasic 1.0g Sodium chloride 5.0g Magnesium sulfate .2g Potassium Phosphate dibasic 1.0g Agar 15g Water 1L
What is the composition of luria agar?
The composition of luria agar is NaCl,Trypotone, Yeast extract and agar.
What ingredient in PEA supplies nitrogen?
PEA agar, also referred to as Phenylethyl Alcohol Agar is a medium used by microbiologists to grow microorganisms. The ingredient that supplies nitrogen is casein.
What is the composition of spirit blue agar?
The composition of spirit blue agar is: casin enzyme hydrolyset 10g yeast extract 5g spiritblue 0.15g agar 15g ph 6.8
Which ingredient in PEA supplies carbon?
Casein is the ingredient in PEA that supplies carbon, as well as nitrogen. PEA is also known as Phenylethyl Alcohol Blood Agar.
Can PEA agar grow in E. coli?
A better way to phrase the question you are asking is "Can PEA agar grow E. coli?" The answer is yes, but only a small amount of growth will be observed because PEA is partially inhibitory to Gram negative organisms and E. coli is Gram negative.
What are the ingredients in PEA that supply carbon?
There are many ingredients in PEA that supply carbon. Some of the ingredients are dextrose, yeast extract, animal tissue, and soy peptone.
What is the composition of modified nutrient glucose agar media?
Modified nutrient glucose agar is composed of agar, water, glucose, extracts, peptone and sodium chloride. Modified nutrient glucose agar allows organisms to grow.
What is the nitrogen source in PEA agar?
Pancreatic digest of casein, papaic digest of soybean meal, beef extract, peptone
Is mannitol salt agar complex or synthetic?
Mannitol Salt Agar Complex is a synthetic medium because it is prepared from pure chemical substances and the exact composition of the medium is known.
How might pea agar inhibit both gram negative and gram positive?
By containing more than 25% phenylethyl alcohol.
What is the chemical composition of malt extract agar?
thats a good question but unforunately i dont now the answer
What is composition of starch agar plate?
The composition of staph 110 agar is: Yeast extract - 2.5 g/L Tryptone - 10.0 g/L Lactose - 2.0 g/L Mannitol - 10.0 g/L Sodium Chloride - 75.0 g/L Dipotassium hydrogen phosphate - 5.0 g/L Gelatin - 30.0 g/L Agar - 15.0 g/L
