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In scientific circles, the streak plate method is considered to be a rapid qualitative isolation method. To be effective, one must reduce the number of organisms in the inoculums by spreading a loop of culture over an agar plate. This ensures that individual cells are properly separated on the surface for the purpose of differentiating various species. The method is as follows: Using a sterile loop, microbes are initially transferred to the plate with one swipe. On the subsequent swipes, the loop is heated in the flame of a Bunsen burner to lessen the population of microbes being transmitted. Streak patterns are also done in via T-streak or by applying the loop to four quadrants of the plate.

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