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A pure culture is one in which only one kind of microbial species is found whereas in mixed culture two or more microbial species formed colonies.

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Could you distinguish between axenic culture and pure culture?

An axenic culture refers to a culture containing only one species of microorganism without any other contaminants present. A pure culture, on the other hand, refers to a culture containing only one specific strain or species of microorganism, but it may still have contaminants present. In essence, axenic culture is free of any contaminants, while a pure culture may have contaminants other than the desired microorganism.


Did you get a pure culture in your subculture and how to know?

Ensuring that your culture is pure is very important to getting accurate results of your gram stain. One way to tell if your culture is pure is to test both the control and the experiment cultures. If you get a different result than the one you were expecting, you might have a corrupted culture. Making sure that you have several items of each is helpful, as well.


What type of culture technique would be used to obtain a pure culture?

To obtain a pure culture, a technique called streak plate method is commonly used. This technique involves streaking a sample on an agar plate in a way that isolates individual colonies, allowing for the growth of pure cultures. Subsequent subculturing from a single isolated colony can help to ensure a pure culture.


Can you determine whether a agar deep culture is pure by visually inspecting it without a microscope?

The short answer is no. Microorganisms are just that - microscopic, and the naked eye can not determine between different species, especially in a broth culture. Maybe on a plate, where the colony shapes, sizes, viscosity, and color can be determined, but a broth culture usually needs to be streaked onto a plate to determine if it is pure or not.


Can a Pure culture prepared from mixed broth or mixed agar slant?

To identify the pure culture the best method is to perform sub culturing and it can be done either by on another agar plate by streak plate method or by pour plate methodThe next is to perform staining which give the difference in morphology if the culture is not pure. But we should perform both the test and then should corelate them because none of them is individually 100% full proof

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Could you distinguish between axenic culture and pure culture?

An axenic culture refers to a culture containing only one species of microorganism without any other contaminants present. A pure culture, on the other hand, refers to a culture containing only one specific strain or species of microorganism, but it may still have contaminants present. In essence, axenic culture is free of any contaminants, while a pure culture may have contaminants other than the desired microorganism.


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