The template strand (DNA) is read by RNA polymerase in the 3'-5' direction.
First, RNA polymerase binds to an A-T rich promoter on the DNA which is upstream from the site of translation. Because A-T bonds are weaker than C-G bonds, the double helix opens up at this point and RNA polymerase begins translation. While translaing the DNA template strand, it creates a complementory mRNA strand and thus the Mrna will read 5'-3' with new bases being added at the 3' end.
The direction of synthesis of the new strand would be in the 5'-3' direction because DNA polymerase can add free nucleotides to only the 3' end of the newly forming strand.
5' to 3'
no DNA synthesis occurs in 5'-3' as well as in 3'-5' direction. only the difference is that ,that it is continuous in 5'-3' direction but discontinous in 3'-5' direction. this is because enzyme DNA polymerase III can synthesize DNA contionously in 5'-3' direction only. ------------------------------------------- No DNA synthesis always occus in the 5' to 3' direction as there is no DNA polymerase that work in the other direction as it is too thermodynamically unfavourable. The cells solve the problem of having to synthesize in a 3' to 5' direction by synthesizing in reverse in discontinuous pieces. Technically there's never any direct 3' to 5' synthesis. -------- ------------------------------------------------- DNA polymerase can add nucleotide to 3' end of primer or growing strand of DNA so in one direction it remains continuous ie leading strand while in in other strand it starts little later when it get enough space to grow in opposite direction then after synthesizing short strand it again goes to newly unwind strand and repeats the process, but the growth remains in 5' to 3' direction moving on template from 3' to 5' direction.
DNA polymerase cannot begin the synthesis of new DNA.To synthesis a new strand of DNA ,RNA primer is required.The complementary RNA nucleotides,that are added opposite to the single strand of parent DNA are the RNA primer.
DNA polymerase catalyzes the reactions that are responsible for synthesizing new DNA strands in the 5' to 3' direction. The parent DNA strand is read in the 3' to 5' direction but the daughter strand is extended in the opposite direction.
I believe it's called replication. not really sure though...
The leading strand is created continuously, but the lagging strand is created as small fragments, known as Okazaki fragments. These fragments are later joined together to form one complete strand.
to add complementary nucleotide respect to the old strand for new strand synthesis.....
Leading strands are one of the two newly synthesized DNA strands during DNA replication. They are synthesized in a continuous manner in the 5' to 3' direction, following the replication fork. The leading strand is synthesized in the same direction as the replication fork is moving, allowing for continuous synthesis.
Following the initiation of DNA replication, the first step is the synthesis of a short RNA primer.
transcription:"the first step in protein synthesis, a sequence of nucleotide bases becomes exposed in an unwound region of a DNA strand. That sequence acts as a template upon which a single strand of RNA - a transcript - is synthesized from free nucleotides."The synthesis of an RNA molecule from the DNA template strand is called transcription.
DNA polymerase
Lagging strand synthesis would be incomplete; leading strand synthesis would be unaffected.
The lagging strand
no DNA synthesis occurs in 5'-3' as well as in 3'-5' direction. only the difference is that ,that it is continuous in 5'-3' direction but discontinous in 3'-5' direction. this is because enzyme DNA polymerase III can synthesize DNA contionously in 5'-3' direction only. ------------------------------------------- No DNA synthesis always occus in the 5' to 3' direction as there is no DNA polymerase that work in the other direction as it is too thermodynamically unfavourable. The cells solve the problem of having to synthesize in a 3' to 5' direction by synthesizing in reverse in discontinuous pieces. Technically there's never any direct 3' to 5' synthesis. -------- ------------------------------------------------- DNA polymerase can add nucleotide to 3' end of primer or growing strand of DNA so in one direction it remains continuous ie leading strand while in in other strand it starts little later when it get enough space to grow in opposite direction then after synthesizing short strand it again goes to newly unwind strand and repeats the process, but the growth remains in 5' to 3' direction moving on template from 3' to 5' direction.
Transcription
DNA polymerase cannot begin the synthesis of new DNA.To synthesis a new strand of DNA ,RNA primer is required.The complementary RNA nucleotides,that are added opposite to the single strand of parent DNA are the RNA primer.
DNA polymerase catalyzes the reactions that are responsible for synthesizing new DNA strands in the 5' to 3' direction. The parent DNA strand is read in the 3' to 5' direction but the daughter strand is extended in the opposite direction.
I believe it's called replication. not really sure though...