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What is the exact role of CO2 in an incubator in an cell culture experiment apart from acting as a buffering agent?
Wiki User
∙ 11y ago
in co2 incubators, the co2 reacts with h2o in the medium to generate hco3- which acts as a buffering system for the cells in the medium. 5% co2 is the optimum concentration for the maintenance of the ph.
Wiki User
∙ 12y ago
Wiki User
∙ 11y agoCO2 in the incubator typically helps to ensure that the buffer works as intended.
Apparently ~5% of CO2 in the air drives typical media towards neutral pH also if they are turning acidic (e.g. because of the cellular metabolism).
In culture media, dissolved CO2 is in equilibrium with bicarbonate ions.CO2 dissolves freely into the culture media and reacts with water to form carbonic acid. As the cells metabolize and produce more CO2, the pH of the medium decreases(becomes more acidic).
Sodium bicarbonate, NaHCO3, is used as a buffer. Sodium bicarbonate dissociates into sodium and bicarbonate ions. By increasing the bicarbonate ions, the buffer drives the top equation to the left and thus increases the pH. The concentration of the sodium bicarbonate in the medium must be matched with the level of CO2 in the atmosphere above the medium.
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