Oil immersion objective lenses are used to further magnify a specimen. The lens requires more light rays to pass through it since it has more mirrors inside, requiring the use of the oil. The oil functions to refract the light rays towards the center of the lens (the normal line of the specimen). By refracting the light towards the center, more rays of light enter the objective, allowing you to see the specimen in a much higher resolution (higher magnification).
The resolving power of lens depends upon its numerical aperture which is equal to product of refrrective index of the medium in which the lens work and sin theta. The refractive index of air is one and sin theta can not be greater then one. So the only way to increase the numerical aperture above one is to increase the refractive index of medium by oil (immersion oil, a colourless liquid with the same refractive index as glass). So to improve resolving power oil is used which replace the air and many rays that did not enter the objective due to reflection and refraction at the surfaces of the object lens and slide will now do so.
When the light passes through the oil's viscous consistency, it creates a beam. :)
It allows for higher resolution images at magnifications of 5000x and higher, i.e., when light refraction becomes a problem.
oil immersible objective-a part of micriscope that helps lense ^^
So that you do not scratch the lens because at 100x magnification it is very very close to the slide.
amboy lang ka ha
100x lens is used with oil immersion
Oil immersion is a technique used to increase the resolution of a microscope.
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Wet mounts are not attached to the slide, it is "floating". If you use an oil immersion lens, you have to put a drop of oil on the slide cover so the light can correctly focus. The oil will cause the slide cover to stick to the end of your Oil immersion lens. When you focus the field of focus will stay the same since the slip cover is stuck to the lens. When you go to change objectives, the cover will travel with your oil immersion lense.
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
100x lens is used with oil immersion
So that you do not scratch the lens because at 100x magnification it is very very close to the slide.
Oil immersion is a technique used to increase the resolution of a microscope.
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
Because that lens (it does not HAVE to be a 100x lens - other lens magnifications are also available in oil immersion constructions) is made to be in contact with a drop of special oil which covers the specimen. The oil has the same index of refraction as the front lens glass and that way a different optical construction can be used.
This is because of the refractive propoties of the immersion oil. The oil works as a sort of secondary lens, magnifying the specimin in addition to the lens alresdy present.
the hanging drop procedure uses a thicker slide, which could crack if the oil immersion lens is used. My bio professor worte: hanging drop slides asre too thick to use under oil.
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Wet mounts are not attached to the slide, it is "floating". If you use an oil immersion lens, you have to put a drop of oil on the slide cover so the light can correctly focus. The oil will cause the slide cover to stick to the end of your Oil immersion lens. When you focus the field of focus will stay the same since the slip cover is stuck to the lens. When you go to change objectives, the cover will travel with your oil immersion lense.
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
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