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How do you determine if a broth is sterile?

Check for any non-granular sediments if the broth was stationary.Check for turbidity in the broth.Turbidity can be checked by tilting the flask towards light and slowly rotating it while keeping an eye on any difference in light transmittance/color/texture/threads/anything that is different from the rest of the broth!If not sure, autoclave it again!!P.S. Turbidity and sediments can only be visible if there was ample incubation, it will NOT be visible at the moment you make it!!Other advanced way can be in the use of spectroscopy. Take fresh broth as blank and suspected broth as sample and check for optical densities. If the suspect one has a greater turbidity than the fresh one then probably your broth is contaminated.P.S. Right wavelength is the key! It should be set according to the color of your broth!Good luck!


Which microscope is use to study internal parts of a cell?

An electron microscope is typically used to study the internal parts of a cell because of its high resolution and magnification capabilities. Transmission electron microscopes (TEMs) and scanning electron microscopes (SEMs) are commonly employed for this purpose.


What term refers to an advantage that men have when employed in female-dominated occupations?

The term "glass escalator" is used to describe an advantage that men often experience in female-dominated occupations, where they have faster promotions and increased opportunities for advancement compared to their female counterparts.


People who represent interest groups and work with legislature are called?

Lobbyists. They advocate on behalf of interest groups to influence government decisions and policies through direct interaction with legislators and officials. Lobbyists can be employed by corporations, advocacy organizations, or other special interest groups.


Which mission is NOT supported by biometrics?

Biometrics are not typically used to support missions related to geological research or environmental conservation. These types of missions usually focus on data collection and analysis rather than personal identification through biometric technology.

Related Questions

What does microbial contamination mean?

Microbial contamination refers to the presence of harmful microorganisms, such as bacteria, viruses, fungi, or parasites, in a substance or environment where they can cause harm. This can occur in food, water, surfaces, or medical equipment, leading to health risks, infections, or spoilage. Preventing microbial contamination is essential in various fields, including food safety, healthcare, and environmental management. Effective hygiene practices and sterilization methods are commonly employed to minimize this risk.


What is clean technique what dose this involve when should this be used?

Clean techniques is the laboratory practices that is employed to reduce the risk of contamination and it should be used in forensic DNA laboratory so as prevent the transfer of the DNA from the analyst to the sample, environment to the sample, and cross-contamination between the samples.


What is cean technique what dose this involve when should this be used?

Clean techniques is the laboratory practices that is employed to reduce the risk of contamination and it should be used in forensic DNA laboratory so as prevent the transfer of the DNA from the analyst to the sample, environment to the sample, and cross-contamination between the samples.


What is the use of canula in laboratory?

A cannula in a laboratory setting is used as a thin, flexible tube for various purposes, such as transferring fluids, gases, or samples between containers. It allows for precise control over the flow and minimizes contamination during procedures. Additionally, cannulas can be employed in experiments requiring the infusion or withdrawal of substances in a controlled manner, enhancing the accuracy and reliability of results.


How do you use a spatula in a laboratory?

In a laboratory, a spatula is used for transferring small amounts of solid chemicals or powders from one container to another. It can also be employed to mix substances, scrape residues from containers, or facilitate the handling of delicate materials without contamination. When using a spatula, it's important to ensure it is clean and appropriate for the materials being handled to avoid cross-contamination. Proper technique helps maintain accuracy and safety in experiments.


What is syringe method?

The syringe method is a technique used in various fields, including medicine and research, to accurately deliver precise volumes of liquids. It involves using a syringe to draw up a specific amount of fluid, allowing for controlled dispensing. This method is commonly employed for administering medications, conducting laboratory experiments, and in procedures requiring precise measurements. Its effectiveness lies in its simplicity and ability to minimize waste and contamination.


technicians?

a person employed to look after technical equipment or do practical work in a laboratory.


What is the definition of technician?

A person employed to look after technical equipment or do practical work in a laboratory.


Sterilization when would this method be used for decontamination purposes?

Sterilization is used for decontamination purposes when there's a need to eliminate all forms of microbial life, including bacteria, viruses, fungi, and spores. This method is crucial in settings such as hospitals, laboratories, and food processing facilities, where ensuring complete sterility is essential to prevent infections and contamination. Common sterilization techniques include autoclaving, dry heat, and chemical sterilants, depending on the materials being treated. It is typically employed for surgical instruments, laboratory equipment, and other items that require a high level of hygiene.


What is the cultivation of microorganisms?

The cultivation of microorganisms involves growing and maintaining microbial organisms, such as bacteria, fungi, and yeasts, under controlled conditions. This process typically requires a suitable medium that provides essential nutrients, optimal temperature, pH, and oxygen levels. Cultivation is essential for various applications, including research, biotechnology, and pharmaceuticals, allowing scientists to study microbial behavior, produce fermented products, or develop antibiotics. Techniques such as aseptic techniques and selective media are often employed to ensure successful and contamination-free growth.


What is surface swabbing?

Surface swabbing is a method used to collect samples from surfaces to test for the presence of contaminants, such as pathogens, chemicals, or allergens. This technique involves using a sterile swab to wipe a specific area, which is then analyzed in a laboratory for microbial growth or chemical detection. It is commonly employed in various fields, including food safety, healthcare, and environmental monitoring, to ensure cleanliness and compliance with safety standards.


What is the sponge method based on?

The sponge method is based on traditional bread making techniques but employed highly mechanized procedures