Turbidity can be checked by tilting the flask towards light and slowly rotating it while keeping an eye on any difference in light transmittance/color/texture/threads/anything that is different from the rest of the broth!
If not sure, autoclave it again!!
P.S. Turbidity and sediments can only be visible if there was ample incubation, it will NOT be visible at the moment you make it!!
Other advanced way can be in the use of spectroscopy. Take fresh broth as blank and suspected broth as sample and check for optical densities. If the suspect one has a greater turbidity than the fresh one then probably your broth is contaminated.
P.S. Right wavelength is the key! It should be set according to the color of your broth!
Good luck!
If you autoclave it for 15 mins at 121oC and at 15lbps pressure then it is called sterlized and to check it just put it in incubator with shaker for 24 hrs it got turbidit due to microbial cells growth then it is said to be not sterile and if there is no cell growth in it then it is sterile
You go and get tested.
The short answer is no. Microorganisms are just that - microscopic, and the naked eye can not determine between different species, especially in a broth culture. Maybe on a plate, where the colony shapes, sizes, viscosity, and color can be determined, but a broth culture usually needs to be streaked onto a plate to determine if it is pure or not.
This definition is only one or the other. Either sterile or non-sterile. There is no such thing as partially sterile.
Sterilized broth is clear, meaning no bacterial growth. Non-sterilized broth is cloudy.
Are mucous membranes sterile
sterile
Showed that sterile broth remained free of microorganisms until exposed to air.
If the pasteur tipped on of his flasks so that the broth came into contact with the curve of the neck, the sterile broth soon became contaminated with microorganisms which were trapped in the curve.
Add the sterile glycerol after growing them in broth. However, -80 freezer preserves them for longer than normal freezer.
Blood type does not determine sterility.
Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
Nutrient broth
The short answer is no. Microorganisms are just that - microscopic, and the naked eye can not determine between different species, especially in a broth culture. Maybe on a plate, where the colony shapes, sizes, viscosity, and color can be determined, but a broth culture usually needs to be streaked onto a plate to determine if it is pure or not.
In a science experiment, a broth is a liquid and a flask is a particular shape of glassware. If the method of your experiment refers to "the broth in the flask", look back over the previous steps and figure out what chemicals and liquids you added to the flask. If the goal of the experiment is to determine what the unknown broth in the flask is you may have to determine it by observing it's physical and chemical traits, be careful with the unknown broth in case it is flammable, poisonous or otherwise dangerous.
Yes! Ask your Gynecologist! Good luck!
The only way to determine if a man or woman is sterile is to have them tested. There are a series of tests that can be done on a sample of the man's sperm to determine if he is sterile. As a rule of thumb this is what a man needs to be considered fertile: 2ml or more of semen 2 million sperm per ml of semen 50% or more of the sperm must be correctly formed 50% or more of the sperm must have good motility
the flower is sterile
If a sterile field becomes "contaminated" with a sterile solution, the field remains sterile.