That entirely depends on what you want the outcome of your experiment to be. If you are looking at the bacterial colonies that occupy soil then yes you want to use a sterile technique so that you dont add any external bacteria to the sample.
If however you simple are investigating the contents of the dirt, and the bacteria that reside in it play little to no role, then you should be safe without a sterile technique.
Aseptic technique refers to how doctors and nurses prevent the spread of infection. The word aseptic means to be free of harmful microorganisms.
aseptic technique are all those activity used to prevent the entry of microorganism.
Aseptic (without infection) techniques protect you and what you are working on. That could be a patient or even a microorganism in a Petri dish.
Its the only way to get a pure culture.
to warm the tube and create warm air convection currents up and away from the opening, to prevent the entrance of dust particles upon which contaminating bacteria reside.
Short answer is Koch's postulates. Long answer you can't ascribe any scientific data you may gather to a bunch of bacteria on a plate or in a culture, as you won't know which of the bacteria gave you the result you observed. So if you are trying to figure out which bacteria causes a disease, inoculating a mix of bacteria won't tell you which one causes the disease. Isolating each one using aseptic technique and then inoculating individually and finding the disease in one animal will tell you which bacteria caused to disease.
There is no "set" time limit. For proper aseptic technique, you should wait until the inoculating loop/needle is visibly red so that all organisms are dead, etc.
sterilization
The terms sterile and aseptic mean the same thing. And you are supposed to use a sterile/aseptic technique. From what I have read, many visiting nurses do not do that if they are from the doctors' office. But they do it in the hospital. It is always best to do this using a technique that will not infect the patient.
Infection Control treatment
Joseph Jackson Lister
dcsddsdsds
Aseptic technique refers to how doctors and nurses prevent the spread of infection. The word aseptic means to be free of harmful microorganisms.
Washing hands is one of the first steps in practicing aseptic technique. Wearing gloves for non sterile procedures is another.
aseptic technique are all those activity used to prevent the entry of microorganism.
Sterialization or aseptic technique
The aseptic technique is performing a procedure using sterile conditions. Aseptic technique was first used around 1847 by Ignaz Semmelweis who suggest to all doctors to wash their hands before and after seeing patients, other doctors dismissed his theory.