It helps position RNA polymerase correctly at the promoter.
The result you get from reading the gel from the bottom up is called the anti-coding (or non-coding strand).
DNA is made of of two complimentary strands, the coding strand and the template strand. When DNA is transcribed (made into messenger RNA which can be converted by ribosomes into proteins) the DNA splits open and free nucleotide bases bind to the template strand. DNA is made of T/C/G/A and RNA is made of U/C/G/A nucleotide bases. G and C bind (they are said to be 'complimentary') A and T bind and in RNA U and A bind (so U replaces T.) The newly formed RNA strand (made on the template stand of DNA) is 'complimentary' to the template but the same as the coding strand of DNA. Hence the template is used to produce RNA which is a copy of the coding strand. Either strand of DNA can act as the template/coding strand. Hope that is a little bit helpful!
The difference between the coding strand and the template strand is the coding strand is the strand which contains the coding genes, i.e. the one in which the RNA polymerase reads and transcribes into mRNA. It must have the promoter sequence in the correct orientation for transcription, as follows:5`-TATAATGCGCGCGCGCGCGCGCGC-3`3`-ATATTACGCGCGCGCGCGCGCGCG-5`In this sequence, the top strand is the coding strand, because it contains the promoter (TATAAT) in the correct orientation.However, when transcribed, the mRNA will be as follows:5`-GCGCGCGCGCGCGCGCGCGC-3`This is because the polymerase transcribes from the template strand, on the opposide side to the coding strand, to make it in the same orientation as the coding strand.I hope I have explained it enough for people to understand, however if I haven't please read this article I found which explains it thoroughly:http://www.bio.net/bionet/mm/bioforum/1994-May/008821.html
AAC CT would produce TTG GA The coding strand is the DNA strand that has the same base sequence as the RNA transcript. It contains codons, and the non-coding strand has anti-codons instead.
You use the mRNA. ;)
According to me,when this strand is transcribed the mRNA formed is not coding for any mino acid that is why this portion of gene is removed from DNA.
AAC CT would produce TTG GA The coding strand is the DNA strand that has the same base sequence as the RNA transcript. It contains codons, and the non-coding strand has anti-codons instead.
"Coding segments" is the term given to genes, segments of the DNA strand that code for a protein. Much of an organism's genome is non-coding segments, portions that do not have a role in protein synthesis.
The result you get from reading the gel from the bottom up is called the anti-coding (or non-coding strand).
When a complementary strand and a coding strand are combined in a test tube the result is a recombinant DNA strand.
It is a copy of the Dna original strand.
DNA fingerprinting identifies and replicates the non-coding gene sequences.
The non-coding side of DNA, also known as the non-coding strand or the template strand, serves as a blueprint for producing RNA molecules during the process of transcription. Unlike the coding strand, which has the same sequence as the RNA product, the non-coding strand has a complementary sequence to the RNA molecule, with the nucleotides A, T, G, and C pairing respectively with U, A, C, and G in RNA.
DNA is made of of two complimentary strands, the coding strand and the template strand. When DNA is transcribed (made into messenger RNA which can be converted by ribosomes into proteins) the DNA splits open and free nucleotide bases bind to the template strand. DNA is made of T/C/G/A and RNA is made of U/C/G/A nucleotide bases. G and C bind (they are said to be 'complimentary') A and T bind and in RNA U and A bind (so U replaces T.) The newly formed RNA strand (made on the template stand of DNA) is 'complimentary' to the template but the same as the coding strand of DNA. Hence the template is used to produce RNA which is a copy of the coding strand. Either strand of DNA can act as the template/coding strand. Hope that is a little bit helpful!
The difference between the coding strand and the template strand is the coding strand is the strand which contains the coding genes, i.e. the one in which the RNA polymerase reads and transcribes into mRNA. It must have the promoter sequence in the correct orientation for transcription, as follows:5`-TATAATGCGCGCGCGCGCGCGCGC-3`3`-ATATTACGCGCGCGCGCGCGCGCG-5`In this sequence, the top strand is the coding strand, because it contains the promoter (TATAAT) in the correct orientation.However, when transcribed, the mRNA will be as follows:5`-GCGCGCGCGCGCGCGCGCGC-3`This is because the polymerase transcribes from the template strand, on the opposide side to the coding strand, to make it in the same orientation as the coding strand.I hope I have explained it enough for people to understand, however if I haven't please read this article I found which explains it thoroughly:http://www.bio.net/bionet/mm/bioforum/1994-May/008821.html
to add complementary nucleotide respect to the old strand for new strand synthesis.....
No, just one strand, the coding strand is used to create a mRNA molecule in the process of transcription.