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Wet mount preparation allows viewing of live and motile microorganisms which would be otherwise killed due to harsh staining procedures
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∙ 12y ago
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What info can you observe on a wet mount that cannot be seen on a stained preparation?
The movement of a bacteria can be seen on a wet mount. This movement can not be seen in a stained preparation.
What specimen preparation is best for viewing cell motility?
hanging drop
Advantages of the wet-mount preparation?
Cells remain moist in a wet mount, allowing you to see the cell components clearly. Specimens appear in their natural condition. It is possible to observe living and moving organisms. It is also possible to observe certain processes of life, such as feeding and cell division (for water-based mounts).
An unstained wet mount preparation of epidermal cells shows few details Which substance could be added to the slides to make the details more visible?
1. methylene blue
What is a wet mounts?
mount thats wet. :trollface:
What info can you observe on a wet mount that cannot be seen on a stained preparation?
The movement of a bacteria can be seen on a wet mount. This movement can not be seen in a stained preparation.
Why a smear preparation is preferred compare to a wet mount?
bcoz a heat-fix smear have a good contrast than the wet mount
What specimen preparation is best for viewing cell motility?
hanging drop
Aside from motility what facts learned from hanging drop preparation?
The hanging drop preparation can study the motility over a longer period of time, but it is more difficult to examine than a wet mount. Wet mount is preferable to a hanging drop slide when using phase contrast optics.
Why would a wet mount be used?
The benefit of a wet mount is that you can view the specimen in its natural environment without killing it. If you do a wet mount you can get proper air around the microbes to view them for a while before they possibly die. If you do a hanging wet mount you have the capability to see them moving in an aerated manner.
What is to mount a slide?
A wet mount slide is when a solution is applied to a microscope slide with whatever subject is being studied, typically under a slip cover.
Why microorganisms hard to see in wet preparation?
In a wet mount the microorganisms are hard to see because they blend in with their surroundings or the liquid they are grown in. An example of this would be in a hay broth where the water is cloudy and the microorganisms are transparent. However, if the microorganisms are being viewed as a stained preparation they will stand out and you will be able to see their size and shape much easier.
Advantages of the wet-mount preparation?
Cells remain moist in a wet mount, allowing you to see the cell components clearly. Specimens appear in their natural condition. It is possible to observe living and moving organisms. It is also possible to observe certain processes of life, such as feeding and cell division (for water-based mounts).
An unstained wet mount preparation of epidermal cells shows few details Which substance could be added to the slides to make the details more visible?
1. methylene blue
What is a wet mounts?
mount thats wet. :trollface:
Which works best with phase contrast microscopy wet mount or hang drop?
wet mount slide
How stain is applied to a slide holding a wet mount preparation?
All you have to do is get a yellow stain and just drop one drop and it should spread throughout the water and should turn that color.
