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bcoz a heat-fix smear have a good contrast than the wet mount

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Ebba Hoeger

Lvl 13
3y ago

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Why a heat-fix smear is preferred compare to a wet mount?

A heat-fix smear is preferred over a wet mount because it allows for the preservation of cellular morphology and enhances the adherence of the specimen to the slide, preventing it from washing away during staining. Additionally, heat-fixing kills the microorganisms, making the sample safer to handle while allowing for clearer visualization of cellular structures under a microscope. This method also facilitates better penetration of stains, resulting in more distinct and reliable results for diagnostic purposes.


What can be observed on a plaque smear wet mount?

difference in plaque smear and yeast smear


Why dense smear not a good smear preparation?

A dense smear will make it difficult to evaluate cells, as they may be all "piled up" and hard to evaluate.


Should you always use sterilized water for smear preparation?

Yes, when you are performing smear preparation, you should always use sterilized water. This will ensure that nothing contaminates your slide.


What is the effect of a very thin preparation in fecal smear?

no sign from the teacher.


Why are thick or dense smears less likely to provide a good smear preparation?

because if too much smear the sample will look to indistinct


What happens to Smudge cells in an albumin smear?

Cell fragility causes smudge cells to appear on peripheral smears. Addition of albumin prior to slide preparation essentially coats the fragile cells, thus keeping them intact on smear preparation.


What is another name for a tzanck preparation?

Tzanck preparation is also called a Tzanck smear, herpes stain for inclusion bodies, or inclusion bodies stain.


Can sterile water use for smear preparation?

Yes, sterile water can be used for smear preparation, particularly in microbiological and laboratory settings. It helps to create a homogeneous specimen and minimizes contamination. However, it's essential to ensure that the sterile water does not interfere with the subsequent analysis or staining processes. Always follow specific protocols for the type of smear being prepared.


Why use dye in preparation of cheek smear specimen?

Dye is used in the preparation of cheek smear specimens to provide contrast and make it easier to visualize and identify cells under the microscope. Dye helps highlight the cellular structures of the cheek cells, making it easier for scientists to analyze and study them.


What are the precautions during preparation of bacterial smear and heat -fixing process?

During the preparation of a bacterial smear, it is crucial to use sterile techniques to avoid contamination. Use a sterile inoculating loop to transfer a small amount of culture onto a clean slide, and ensure the smear is thin and evenly spread. When heat-fixing, gently pass the slide through a flame to adhere the bacteria to the slide without burning them, as excessive heat can distort cellular structures. Always wear gloves and goggles to protect yourself from potential biohazards.


What is another name for a slide that has no liquid or cover slip?

A dry mount slide is a slide that has no liquid or cover slip. This type of slide is commonly used for observing solid specimens under a microscope.