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What dye is used to stain cheek epithelial cells in lab?
methane blue
Why is it you should heat-fixing the air-dried before staining?
It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.
What is the purpose of negative staining experiment?
Answer by NO.1GreatThinker :- The basic purpose of the negative staining experiment is to check out the bacteria / micro-organisms which have less amount of peptidoglycans present in their cell wall So experiment can be performed to distinguish gram negative bacteria from gram positive one . It is performed by using a dye nigrosin which is a negatively charged dye in nature . So it is first taken on a slide ( just a drop of it ) . Then specimen of the bacteria is taken from the culture . It is placed over the dye . Now since bacteria is also negatively charged . So there is repulsion in both dye and the bacteria . Hence the bacteria seems or appears in between the dye as bright light spots . It is observed under the light microscope . Hence the experiment .
What is the effect of pH in the preparation of dye?
The effect of it is so that the person doing the experiment knows exactly if the chemical is acidic or alkeline.
What colour are cheek cells?
methelyn blue
What is it called to add dye to a specimen under a microscope?
to stain.
What dye is used to stain cheek epithelial cells in lab?
methane blue
What is active ingredient in the dye preparation that will lighten your hair?
Hydrogen peroxide
Why is it you should heat-fixing the air-dried before staining?
It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.
What is the preparation of smears and simple staining?
smear is the putting and fixing of staining sample on glass slide which is done by first putting the a drop of water on slide and then inoculation is put over it which is then spread slowly in round form by inoculating loop and dry it by very light heat to fix it.Simple staining is the process in which a dye knwon as methylene blue is spread over smear to colour the microbe whcih can be then washed by 70% alcohol so that extra dye can be removed and then the sample is ready to observe under microscope
Uses of cover slip?
A cover slip is used in microscopy to flatten and protect the specimen, to keep the microscope lens from touching the specimen directly, and to prevent the specimen from drying out. It also helps improve the resolution and clarity of the observed sample under the microscope.
What is the purpose of negative staining experiment?
Answer by NO.1GreatThinker :- The basic purpose of the negative staining experiment is to check out the bacteria / micro-organisms which have less amount of peptidoglycans present in their cell wall So experiment can be performed to distinguish gram negative bacteria from gram positive one . It is performed by using a dye nigrosin which is a negatively charged dye in nature . So it is first taken on a slide ( just a drop of it ) . Then specimen of the bacteria is taken from the culture . It is placed over the dye . Now since bacteria is also negatively charged . So there is repulsion in both dye and the bacteria . Hence the bacteria seems or appears in between the dye as bright light spots . It is observed under the light microscope . Hence the experiment .
What is the effect of pH in the preparation of dye?
The effect of it is so that the person doing the experiment knows exactly if the chemical is acidic or alkeline.
What is the function of ammonium chloride in preparation of martius yellow?
wash the product.The work of ammonium chloride in preparation of martius yellow is to polarize the compound structure of the solution.this allows exchange of groups during dyeing.it also helps in formation of soluble salt which can be used as a dye for wool and other materials.
What colour are cheek cells?
methelyn blue
Is a coverslip used during negative staining?
No, a coverslip is not typically used during negative staining. In negative staining, the specimen is mixed with a contrast dye that stains the background rather than the specimen itself, allowing the cells to stand out against the dark background. This technique is often performed directly on a microscope slide without a coverslip to preserve the morphology and details of the specimen.
Why might a scientist use colored dye when viewing items under a compound light microscope?
A scientist might use colored dye to enhance the contrast and make specific structures or features more visible. Dye can help highlight certain parts of a specimen, making it easier to observe and study under a compound light microscope.
