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What dye is used to stain cheek epithelial cells in lab?

methane blue


Why is it you should heat-fixing the air-dried before staining?

It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.


When preparing a wet mount of cheek cells why is it important not to overstain your preparation?

When preparing a wet mount of cheek cells, it is important not to overstain because excessive dye can obscure cellular details and structures, making it difficult to observe and identify specific features. Overstaining can also lead to a lack of contrast, which hampers the visibility of the cells under a microscope. Additionally, an overly stained sample may affect the overall clarity and accuracy of the observations.


What is the purpose of negative staining experiment?

Answer by NO.1GreatThinker :- The basic purpose of the negative staining experiment is to check out the bacteria / micro-organisms which have less amount of peptidoglycans present in their cell wall So experiment can be performed to distinguish gram negative bacteria from gram positive one . It is performed by using a dye nigrosin which is a negatively charged dye in nature . So it is first taken on a slide ( just a drop of it ) . Then specimen of the bacteria is taken from the culture . It is placed over the dye . Now since bacteria is also negatively charged . So there is repulsion in both dye and the bacteria . Hence the bacteria seems or appears in between the dye as bright light spots . It is observed under the light microscope . Hence the experiment .


What is the effect of pH in the preparation of dye?

The effect of it is so that the person doing the experiment knows exactly if the chemical is acidic or alkeline.

Related Questions

What is it called to add dye to a specimen under a microscope?

to stain.


What dye is used to stain cheek epithelial cells in lab?

methane blue


What dye do we use to dye cheek cells?

To dye cheek cells, methylene blue is commonly used. This dye stains the cell nuclei and helps to visualize the cells under a microscope. It enhances the contrast between the cells and their background, making it easier to observe cellular structures. Other dyes, such as iodine or Giemsa stain, can also be used depending on the specific needs of the observation.


What is active ingredient in the dye preparation that will lighten your hair?

Hydrogen peroxide


Why is it you should heat-fixing the air-dried before staining?

It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.


What is the preparation of smears and simple staining?

smear is the putting and fixing of staining sample on glass slide which is done by first putting the a drop of water on slide and then inoculation is put over it which is then spread slowly in round form by inoculating loop and dry it by very light heat to fix it.Simple staining is the process in which a dye knwon as methylene blue is spread over smear to colour the microbe whcih can be then washed by 70% alcohol so that extra dye can be removed and then the sample is ready to observe under microscope


Uses of cover slip?

A cover slip is used in microscopy to flatten and protect the specimen, to keep the microscope lens from touching the specimen directly, and to prevent the specimen from drying out. It also helps improve the resolution and clarity of the observed sample under the microscope.


When preparing a wet mount of cheek cells why is it important not to overstain your preparation?

When preparing a wet mount of cheek cells, it is important not to overstain because excessive dye can obscure cellular details and structures, making it difficult to observe and identify specific features. Overstaining can also lead to a lack of contrast, which hampers the visibility of the cells under a microscope. Additionally, an overly stained sample may affect the overall clarity and accuracy of the observations.


What is the purpose of negative staining experiment?

Answer by NO.1GreatThinker :- The basic purpose of the negative staining experiment is to check out the bacteria / micro-organisms which have less amount of peptidoglycans present in their cell wall So experiment can be performed to distinguish gram negative bacteria from gram positive one . It is performed by using a dye nigrosin which is a negatively charged dye in nature . So it is first taken on a slide ( just a drop of it ) . Then specimen of the bacteria is taken from the culture . It is placed over the dye . Now since bacteria is also negatively charged . So there is repulsion in both dye and the bacteria . Hence the bacteria seems or appears in between the dye as bright light spots . It is observed under the light microscope . Hence the experiment .


What is the effect of pH in the preparation of dye?

The effect of it is so that the person doing the experiment knows exactly if the chemical is acidic or alkeline.


What is the function of ammonium chloride in preparation of martius yellow?

wash the product.The work of ammonium chloride in preparation of martius yellow is to polarize the compound structure of the solution.this allows exchange of groups during dyeing.it also helps in formation of soluble salt which can be used as a dye for wool and other materials.


What colour are cheek cells?

methelyn blue